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Invitrogen

EGF Monoclonal Antibody (3A8), Biotin

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Product Details

M806B

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Immunocytochemistry (ICC/IF)

1:50
-

ELISA (ELISA)

0.125-0.25 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3A8

Immunogen

Recombinant Human EGF

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536658

Product Specific Information

The M806B anti-EGF antibody (biotinylated conjugate of clone 3A8) has successfully been paired as the detection antibody in a sandwich ELISA with coating antibody M805 (Clone 1H11).

Typical dilutions for sandwich ELISA: Coat = 1-3 µg/mL and Detection = 0.125-0.5 µg/mL.

Target Information

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta-urogastrone; EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor; unnamed protein product

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Gene Aliases: EGF; HOMG4; URG

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UniProt ID: (Human) P01133

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Entrez Gene ID: (Human) 1950

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Function(s)
guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding calcium ion binding protein binding growth factor activity transmembrane receptor protein tyrosine kinase activator activity receptor agonist activity
Process(es)
angiogenesis positive regulation of endothelial cell proliferation positive regulation of receptor internalization epidermal growth factor receptor signaling pathway cell proliferation positive regulation of cell proliferation positive regulation of endothelial cell migration positive regulation of macromolecule metabolic process positive regulation of gene expression positive regulation of peptidyl-threonine phosphorylation cerebellar granule cell precursor proliferation positive regulation of cerebellar granule cell precursor proliferation positive regulation of cell migration ERBB2-EGFR signaling pathway positive regulation of phosphorylation positive regulation of DNA binding positive regulation of MAPK cascade positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated regulation of JAK-STAT cascade system development branching morphogenesis of an epithelial tube epithelial cell proliferation positive regulation of epithelial cell proliferation negative regulation of secretion regulation of transport positive regulation of protein kinase B signaling mammary gland alveolus development ERK1 and ERK2 cascade regulation of primary metabolic process positive regulation of canonical Wnt signaling pathway regulation of calcium ion import negative regulation of cholesterol efflux positive regulation of hyaluronan biosynthetic process positive regulation of protein localization to early endosome positive regulation of epithelial tube formation regulation of protein localization to cell surface positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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