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Invitrogen
alpha Adaptin Monoclonal Antibody (AP6)
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FIGURE: 1 / 15
alpha Adaptin Antibody (MA1-064) in ICC/IF
Immunofluorescence analysis of alpha Adaptin was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Adaptin (AP6) Mouse Monoclonal Antibody (Product # MA1-064) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-064
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Published species
Bovine,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
AP6
Immunogen
Purified adaptors.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-064 detects assembly polypeptide 2 (AP2) from a wide variety of mammalian sources, including human, hamster, monkey, bovine, rat, and mouse. It recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha adaptin found in neurons.
MA1-064 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~100 kDa doublet representing the two isoforms of alpha adaptin in rat brain (alpha C is the dominant form seen)
Microinjection of cells with MA1-064 results in variable blockade of endocytosis.
The MA1-064 immunogen is purified adaptors.
Target Information
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; adaptor protein complex AP-2 subunit alpha-1; adaptor protein complex AP-2, alpha 1 subunit; adaptor related protein complex 2 alpha 1 subunit; adaptor-related protein complex 2 subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; adaptor-related protein complex AP-2, alpha 1 subunit; ADTAB; alpha-adaptin A; alpha-adaptin C; alpha1-adaptin; alpha2-adaptin; AP-2 complex subunit alpha-1; AP2; CLAPA2; clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; HIP-9; HIP9; HYPJ; plasma membrane adaptor HA2/AP2 adaptin alpha A subunit; unnamed protein product
Gene Aliases: ADTAA; AP2-ALPHA; CLAPA1
UniProt ID: (Human) O95782, (Mouse) P17426
Entrez Gene ID: (Human) 160, (Bovine) 784636, (Chinese hamster) 100772481, (Rat) 308578, (Mouse) 11771
intracellular protein transport
Golgi to endosome transport
endocytosis
protein transport
vesicle-mediated transport
synaptic vesicle endocytosis
clathrin-mediated endocytosis
postsynaptic neurotransmitter receptor internalization
negative regulation of hyaluronan biosynthetic process
positive regulation of neuron projection development
positive regulation of receptor-mediated endocytosis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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