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Invitrogen
CD229 Monoclonal Antibody (HLy9.25)
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FIGURE: 1 / 2
CD229 Antibody (MA1-10149) in Flow
Separation of human CD229 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD229 (HLy9.25) purified Monoclonal antibody (Product # MA1-10149); concentration in sample 16 µg/mL, GAM APC.
Product Details
MA1-10149
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
HLy9.25
Immunogen
CD299-transfected 300.19 pre-B cell line
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.
Target Information
CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD229; Cell surface molecule Ly-9; Ly-9; Lymphocyte antigen 9; RP11-312J18.1; Signaling lymphocytic activation molecule 3; similar to Homo sapiens lymphocyte antigen 9 precursor (LY-9) mRNA with GenBank Accession Number AF55709; SLAM family member 3; SLAMF3; T-lymphocyte surface antigen Ly-9; unnamed protein product
Gene Aliases: CD229; CDABP0070; hly9; LY9; mLY9; SLAMF3
UniProt ID: (Human) Q9HBG7
Entrez Gene ID: (Human) 4063
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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