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Invitrogen
CD44 Monoclonal Antibody (IM7)
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FIGURE: 1 / 10
CD44 Antibody (MA1-10225) in ICC/IF
Immunofluorescence analysis of CD44 was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4 % paraformaldehyde for 10 minutes, permeabilized with 0.1 % Triton™ X-100 for 15 minutes, and blocked with 2 % BSA for 1 hour at room temperature. The cells were labeled with CD44 Monoclonal Antibody (IM7) (Product # MA1-10225, 10 µg/mL) in 0.1 % BSA incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A48269, 1:... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-10225
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Horse,
Cat,
Human,
Mouse
Published species
Dog,
Human,
Mouse,
Pig
Host/Isotype
Rat
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
IM7
Immunogen
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with all isoforms of mouse CD44.
Western Blot: non-reducing conditions.
Target Information
CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cd44; CD44 antigen-like protein; CD44 molecule; CD44 molecule (Indian blood group); CD44 precursor; CD44R1; CDw44 antigen precursor; cell surface glycoprotein CD44; cell-cell and cell-matrix adhesion interaction glycoprotein isoform; cell-surface glycoprotein; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; epican; extracellular matrix receptor III; extracellular matrix receptor-III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; Hermes antigen; Hermes antigen gp90 homing receptor precursor; homing cell adhesion molecule; homing function and Indian blood group system; homing receptor; HUTCH-I; hyaluronan receptor; hyaluronan-binding protein; hyaluronate receptor; In(Lu) related-p80; Indian blood group antigen; lymphocyte antigen 24; lymphocyte surface antigen precursor CD44; PGP I; PGP-1; PGP-I; PGPI; phagocyte glycoprotein 1; phagocytic glycoprotein 1; phagocytic glycoprotein I; phosphoglycoprotein Pgp-1 (Ly24.2) precursor; sCD 44; sCD44; soluble CD 44; soluble CD44; unnamed protein product
Gene Aliases: CDW44; CSPG8; ECM-III; ECMR-III; H-CAM; HCELL; HERMES; Hermes-1; HUTCH-1; HUTCH-I; LHR; Ly-24; MC56; MDU2; MDU3; MIC4; Pgp-1; Pgp1
UniProt ID: (Human) P16070, (Mouse) P15379
Entrez Gene ID: (Horse) 100034221, (Dog) 403939, (Human) 960, (Mouse) 12505
transmembrane signaling receptor activity
cytokine receptor activity
protein binding
collagen binding
hyaluronic acid binding
signaling receptor activity
cargo receptor activity
type II transforming growth factor beta receptor binding
epidermal growth factor receptor binding
channel regulator activity
protein kinase binding
cytokine binding
phosphoprotein binding
transmembrane signal receptor
cytokine-mediated signaling pathway
wound healing, spreading of cells
regulation of lamellipodium morphogenesis
endocytosis
inflammatory response
cell adhesion
cell-matrix adhesion
anatomical structure morphogenesis
cell migration
hyaluronan catabolic process
positive regulation of heterotypic cell-cell adhesion
T cell activation
regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
cellular response to fibroblast growth factor stimulus
system development
NK T cell activation
cartilage development
positive regulation of ERK1 and ERK2 cascade
monocyte aggregation
cell-cell adhesion
positive regulation of monocyte aggregation
carbohydrate derivative transport
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of cell growth
branching involved in ureteric bud morphogenesis
wound healing involved in inflammatory response
positive regulation of adaptive immune response
negative regulation of mature B cell apoptotic process
receptor-mediated endocytosis
positive regulation of gene expression
Wnt signaling pathway
neuron projection development
positive regulation of neutrophil apoptotic process
macrophage fusion
negative regulation of regulatory T cell differentiation
negative regulation of inflammatory response
branching involved in prostate gland morphogenesis
regulation of modification of postsynaptic structure
postsynapse organization
regulation of leukocyte apoptotic process
negative regulation of CD4-positive, alpha-beta T cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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