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Invitrogen
Nestin Monoclonal Antibody (10C2), Alexa Fluor™ 555
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FIGURE: 1 / 2
Nestin Antibody (MA1-110-A555) in ICC/IF
Immunofluorescent analysis of Nestin (red) in U2OS cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were stained with a Nestin mouse monoclonal antibody (Product # MA1-110-A555) at a concentration of 20 µg/mL in blocking buffer for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific Evos FL Auto Imaging system at 20X magni... View More
Product Details
MA1-110-A555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
10C2
Immunogen
Human nestin protein corresponding to residues 1464-1614
Conjugate
Excitation/Emission Max
553/568 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS with 1.0mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-110 has been successfully utilized in Western Blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ21841; intermediate filament protein; Nestin; unnamed protein product
Gene Aliases: AA166324; C78523; ESTM46; Nbla00170; NES
UniProt ID: (Human) P48681, (Mouse) Q6P5H2
Entrez Gene ID: (Human) 10763, (Mouse) 18008
G2/M transition of mitotic cell cycle
nervous system development
central nervous system development
brain development
positive regulation of intermediate filament depolymerization
embryonic camera-type eye development
negative regulation of protein binding
negative regulation of catalytic activity
negative regulation of neuron apoptotic process
cell projection morphogenesis
neuron apoptotic process
stem cell proliferation
positive regulation of neural precursor cell proliferation
multicellular organism development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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