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Invitrogen

CD45RA Monoclonal Antibody (MEM-56)

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CD45RA Monoclonal Antibody (MEM-56)

Product Details

MA1-19113

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL
-

Flow Cytometry (Flow)

0.3-4 µg/mL
View 22 publications 22 publications

Immunoprecipitation (IP)

Assay-dependent
-

Miscellaneous PubMed (Misc)

-
View 17 publications 17 publications
Product Specifications

Species Reactivity

Human

Published species

Baboon, Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

MEM-56

Immunogen

Human thymocytes and T lymphocytes.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1072449

Product Specific Information

This antibody reacts with an extracellular epitope of CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.

This antibody has been successfully used in Mass Cytometry (CyTof).

Immunohistochemistry (Paraffin): No pre-treatment of tissue sections is needed.

Target Information

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD45; CD45 antigen; glycoprotein 180; L-CA; Leukocyte common antigen; Ly-5; Lyt-4; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein; T200 leukocyte common antigen; unnamed protein product

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Gene Aliases: B220; CD45; CD45R; GP180; IMD105; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)
phosphoprotein phosphatase activity protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity receptor binding protein binding heparin binding hydrolase activity protein kinase binding protein tyrosine kinase inhibitor activity ankyrin binding spectrin binding heparan sulfate proteoglycan binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade natural killer cell differentiation negative regulation of T cell mediated cytotoxicity positive regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation positive regulation of immunoglobulin production positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration leukocyte cell-cell adhesion signal transduction cell surface receptor signaling pathway response to gamma radiation regulation of gene expression dephosphorylation B cell differentiation T cell differentiation positive regulation of B cell proliferation regulation of interleukin-8 production negative regulation of interleukin-2 production positive regulation of interleukin-2 production positive regulation of tumor necrosis factor production heterotypic cell-cell adhesion T cell proliferation B cell proliferation positive regulation of T cell proliferation T cell activation gamma-delta T cell differentiation positive regulation of MAPK cascade cell cycle phase transition plasma membrane raft distribution positive thymic T cell selection negative thymic T cell selection positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of protein kinase activity negative regulation of JAK-STAT cascade alpha-beta T cell proliferation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes bone marrow development stem cell development regulation of phagocytosis positive regulation of phagocytosis positive regulation of calcium-mediated signaling T cell receptor signaling pathway B cell receptor signaling pathway regulation of T cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of Fc receptor mediated stimulatory signaling pathway negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade extrinsic apoptotic signaling pathway negative regulation of interleukin-4-mediated signaling pathway negative regulation of microglial cell activation response to aldosterone DN2 thymocyte differentiation positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway
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