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Invitrogen
GAPDHS Monoclonal Antibody (Hs-8)
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FIGURE: 1 / 2
GAPDHS Antibody (MA1-19235) in Flow
Flow Cytometry analysis of GAPDHS using GAPDHS Monoclonal Antibody (Hs-8) (Product # MA1-19235). Separation of human GAPDHS positive cells (red-filled) from GAPDHS negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human sperm cells stained using anti-GAPDHS (Hs-8) purified antibody (concentration in sample 7.5 μg/mL) GAM APC.
Product Details
MA1-19235
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
Hs-8
Immunogen
Freshly ejaculated human sperms.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
TBS, pH 8
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
For ICC staining, membrane permeabilization with acetone is essential.
Purity is >95% by SDS-PAGE.
Target Information
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory protein Li 278; GAPDH S; GAPDH-2; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; unnamed protein product
Gene Aliases: GAPD2; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-278; HSD-35; HSD35
UniProt ID: (Human) O14556
Entrez Gene ID: (Pig) 100517097, (Human) 26330
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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