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          • Primary Antibodies ›
          • Beta III Tubulin Antibodies

          Invitrogen

          beta-3 Tubulin Monoclonal Antibody (TU-20), FITC

          1 Reference
          View all (49) Beta III Tubulin antibodies

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          Cite beta-3 Tubulin Monoclonal Antibody (TU-20), FITC

          Additional Information:
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          • Antibody Testing Data (2)
          beta-3 Tubulin Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          beta-3 Tubulin Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          beta-3 Tubulin Antibody (MA1-19582) in Flow

          Separation of MCF-7 cells stained using anti-human betaIII-Tubulin (TU-20) FITC Monoclonal antibody (Product # MA1-19582), 10 µL reagent per million cells in 100 µL of cell suspension, red-filled) from MCF-7 cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 15 µg/mL, same as betaIII-Tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of MCF-7 cell suspension. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          beta-3 Tubulin Antibody in Flow Cytometry (Flow)
          beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta-3 Tubulin Monoclonal Antibody (TU-20), FITC

          Product Details

          MA1-19582

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          -

          Flow Cytometry (Flow)

          1-3 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Many, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          TU-20

          Immunogen

          Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
          View immunogen

          Conjugate

          FITC FITC FITC
          • Unconjugated
          • Request custom conjugation

          Excitation/Emission Max

          498/517 nm View spectra spectra

          Form

          Liquid

          Concentration

          1.0 mg/mL

          Amount

          100 µg

          Purification

          Size-exclusion chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          15mM sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1070729

          Product Specific Information

          This antibody recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

          Target Information

          The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: beta 3 tubulin; beta tubulin 3; betaIII-tubulin; class III beta-tubulin; MC1R; microtubule protein; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB; tubulin beta; Tubulin beta-3; Tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3; unnamed protein product

          View more View less

          Gene Aliases: 3200002H15Rik; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; M(beta)3; M(beta)6; TUBB3; TUBB4

          View more View less

          UniProt ID: (Human) Q13509, (Mouse) Q9ERD7, (Rat) Q4QRB4

          View more View less

          Entrez Gene ID: (Human) 10381, (Mouse) 22152, (Rat) 246118

          View more View less

          Function(s)
          nucleotide binding GTPase activity structural constituent of cytoskeleton protein binding GTP binding peptide binding metal ion binding netrin receptor binding tubulin
          Process(es)
          microtubule cytoskeleton organization mitotic cell cycle microtubule-based process axon guidance neuron differentiation netrin-activated signaling pathway dorsal root ganglion development mitotic nuclear division protein polymerization metabolic process
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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