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Invitrogen
CD31 Monoclonal Antibody (MEM-05), PE
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FIGURE: 1 / 4
CD31 Antibody (MA1-19769) in Flow
Knockout of CD31 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR616280_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD31 was performed by staining THP-1 CD31 Knock out cells with 0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-42, yellow histogram) or 0.25 µg CD31 Monoclonal Antibody (MEM-05), PE (Product # MA1-19769, blue histogram). THP-1 Cas9 control cells were also stained with CD31 Monoclonal... View More
Product Details
MA1-19769
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MEM-05
Immunogen
Leukocytes of patient suffering from LGL-type leukaemia.
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with an extracellular epitope of CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adhesion molecule; CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; PECAM-1 precursor; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; unnamed protein product
Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1
UniProt ID: (Human) P16284
Entrez Gene ID: (Human) 5175
angiogenesis
blood vessel development
endothelial cell development
endothelial cell morphogenesis
phagocytosis
immune response
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
leukocyte cell-cell adhesion
signal transduction
cell surface receptor signaling pathway
Rho protein signal transduction
cell recognition
cell migration
regulation of cell migration
positive regulation of cell migration
maintenance of blood-brain barrier
monocyte extravasation
wound healing
vasodilation
positive regulation of MAPK cascade
endothelial cell migration
diapedesis
detection of mechanical stimulus
positive regulation of protein kinase B signaling
establishment of endothelial barrier
bicellular tight junction assembly
cellular response to mechanical stimulus
glomerular endothelium development
neutrophil extravasation
endothelial cell-matrix adhesion
cell-cell adhesion
cell-cell adhesion via plasma-membrane adhesion molecules
positive regulation of protein localization to cell-cell junction
positive regulation of intracellular signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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