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Invitrogen
CD44 Monoclonal Antibody (KM81)
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FIGURE: 1 / 5
CD44 Antibody (MA1-70080) in IHC (P)
Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded mouse spleen sections at 400x magnification with DAPI counterstain. Primary antibody used was a CD44/H-CAM monoclonal antibody (clone KM81) at a 1:100 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-rat at a 1:500 dilution and Normal Goat Serum (blocking)
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-70080
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
KM81
Immunogen
Bone Marrow Derived Stromal Cells (clone BMS2).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
250 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-70080 detects CD44 in mouse samples.
MA1-70080 has been successfully used in flow cytometry procedures.
The MA1-70080 immunogen is bone marrow derived stromal cells (clone BMS2).
The molecular weight of CD44 is 95 kDa.
Store at 4°C short term; store at -20°C long term, avoiding freeze-thaw cycles.
Target Information
CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cd44; CD44 molecule; CD44 precursor; ECMR-III; ECMRIII; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hermes antigen; HUTCH-I; hyaluronan receptor; hyaluronate receptor; lymphocyte antigen 24; PGP I; PGP-I; PGPI; phagocytic glycoprotein 1; phagocytic glycoprotein I; phosphoglycoprotein Pgp-1 (Ly24.2) precursor; sCD 44; sCD44; soluble CD 44; unnamed protein product
Gene Aliases: HERMES; Ly-24; Pgp-1
UniProt ID: (Mouse) P15379
Entrez Gene ID: (Mouse) 12505
transmembrane signaling receptor activity
cytokine receptor activity
type II transforming growth factor beta receptor binding
epidermal growth factor receptor binding
protein binding
hyaluronic acid binding
channel regulator activity
protein kinase binding
cytokine binding
signaling receptor activity
cargo receptor activity
phosphoprotein binding
regulation of cell growth
branching involved in ureteric bud morphogenesis
wound healing involved in inflammatory response
positive regulation of adaptive immune response
negative regulation of mature B cell apoptotic process
receptor-mediated endocytosis
inflammatory response
cell adhesion
anatomical structure morphogenesis
positive regulation of gene expression
Wnt signaling pathway
cell migration
cytokine-mediated signaling pathway
hyaluronan catabolic process
neuron projection development
positive regulation of neutrophil apoptotic process
positive regulation of heterotypic cell-cell adhesion
macrophage fusion
T cell activation
negative regulation of apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
wound healing, spreading of cells
cellular response to fibroblast growth factor stimulus
negative regulation of regulatory T cell differentiation
system development
negative regulation of inflammatory response
NK T cell activation
branching involved in prostate gland morphogenesis
positive regulation of ERK1 and ERK2 cascade
monocyte aggregation
regulation of modification of postsynaptic structure
postsynapse organization
positive regulation of monocyte aggregation
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of leukocyte apoptotic process
regulation of lamellipodium morphogenesis
negative regulation of CD4-positive, alpha-beta T cell proliferation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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