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Invitrogen
SNTB2 Monoclonal Antibody (1351)
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FIGURE: 1 / 22
SNTB2 Antibody (MA1-745) in ICC/IF
Immunofluorescence analysis of Syntrophin was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Syntrophin (1351) Mouse Monoclonal Antibody (Product # MA1-745) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28... View More
Product Details
MA1-745
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Amphibian,
Dog,
Chicken,
Human,
Mouse,
Rat
Published species
Amphibian,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1351
Immunogen
Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-745 detects alpha 1, beta 1 and beta 2 subunits of syntrophin. This antibody has been shown to react with human, mouse, rat, chicken, canine and amphibian samples.
MA1-745 has been successfully used in Western blot, immunoprecipitation and immunofluorescence procedures. By Western blot, this antibody detects an ~58 kDa protein representing syntrophin from rat and mouse cell extracts.
The MA1-745 immunogen is whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane. Characterization studies of this antibody have demonstrated that clone 1351 is directed against an epitope within the PDZ domain of syntrophin. MA1-745, clone 1351, is seen as the "gold standard" for syntrophin assessment.
Target Information
Syntrophins are structurally related proteins located at the neuromuscular synapse and play an important role in dystrophin associated protein (DAP) complex formation. To date there have been three distinct syntrophin isoforms discovered: alpha 1, beta 1, and beta 2. These proteins each contain a PDZ domain, a syntrophin-unique domain (SU), and three pleckstrin homology domains (PH1a,1b and 2). The PDZ domain has been shown to be responsible for binding syntrophin to nNOS and the DAP complex. This association results in localization of the nicotinic acetylcholine receptor to the cytoskeleton via the (S/T)XV C terminus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 59 kDa dystrophin-associated protein A1 basic component 2; Beta-2-syntrophin; dystrophin-associated protein A1, 59kD, basic component 2; SNT3; SNTL; SYN; syntrophin, basic 2; syntrophin, beta 2 (dystrophin-associated protein A1, 59kDa, basic component 2); Syntrophin-3; Syntrophin-like
Gene Aliases: D16S2531E; EST25263; Snt2; SNT2B2; SNT3; SNTB2; SNTL
UniProt ID: (Human) Q13425, (Mouse) Q61235
Entrez Gene ID: (Human) 6645, (Chicken) 769654, (Dog) 479675, (Mouse) 20650, (Rat) 689421
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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