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Invitrogen
CD11b/c Monoclonal Antibody (OX-42), Biotin
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Product Details
MA1-80232
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OX-42
Immunogen
Rat peritoneal macrophages.
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Rat CD11b, clone OX-42, recognizes rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats.
Target Information
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. CD11b is also a receptor for fibrogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% thymocytes and is commonly used as a microgial marker in nervous tissue. CD11c encodes the integrin alpha X chain protein. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Mutations affecting the CD11b/c gene lead to systemic lupus erythematosus 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD11bc; CR3A; Integrin alpha M; integrin, alpha M; integrin, alpha X; MAC-1; MAC1A; MGC117044; MO1A; OX-42; OX42; SLEB6
Gene Aliases: Cd11b; RGD1561123
Entrez Gene ID: (Rat) 25021, (Rat) 499271
phagocytosis
cell adhesion
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
positive regulation of cell proliferation
positive regulation of gene expression
positive regulation of cell migration
positive regulation of myelination
cell-cell adhesion mediated by integrin
heterotypic cell-cell adhesion
positive regulation of angiogenesis
defense response to virus
cell-cell adhesion
positive regulation of endothelial tube morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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