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CD204 Monoclonal Antibody (2F8), FITC
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FIGURE: 1 / 4
CD204 Antibody (MA1-81060) in WB
Proceedings of the National Academy of Sciences of the United States of America 2013 -
Fig. 6. Correlation between uptake of SNAs and expression level of CAV-1 and SR-A. ( A ) To probe the general importance of CAV-1 and SR-A, we selected three other cell lines besides C166 for additional investigation. They include HaCaT (immortal human keratinocyte), 3T3 (mouse fibroblast), and A549 (human lung epithelial adenocarcinoma). All are responsive to the treatment of FCD and Poly I, as evidenced b... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-81060
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fish,
Mouse,
Pig
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2F8
Immunogen
RAW264 cell line.
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Rat anti Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SRA), type I and II, also known as CD204.
Target Information
CD204 gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. Diseases associated with MSR1 include Barrett Esophagus and Prostate Cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: macrophage acetylated LDL receptor I and II; scavenger receptor type A; SR-AI/II; unnamed protein product
Gene Aliases: MRS-A; MSR; MSR-A; Scara1; Scvr; SR-A; SR-AI; SR-AII
UniProt ID: (Mouse) P30204
Entrez Gene ID: (Pig) 100511665, (Mouse) 20288
endocytosis
receptor-mediated endocytosis
phagocytosis, engulfment
negative regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
positive regulation of cholesterol storage
cholesterol transport
positive regulation of toll-like receptor 9 signaling pathway
plasma lipoprotein particle clearance
lipoprotein transport
beta-amyloid clearance
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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