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Invitrogen
Importin beta-1 Monoclonal Antibody (3E9)
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Product Details
MA3-070
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Mouse,
Pig,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
3E9
Immunogen
Purified NTF97 from bovine erythrocytes.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-070 detects Importin beta 1 from human, canine, bovine, rat and mouse tissues as well as porcine cell lysates. Importin beta 1 is also known as NTF97, Nuclear Transport Factor p97, and karyopherin beta 1. This antibody does not detect bacterially expressed recombinant NTF97.
MA3-070 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects a single ~97 kDa protein representing NTF97 in MDBK cell homogenate. Immunofluorescence staining of NTF97 in MDBK cells with MA3-070 results in the labeling of both the nuclear envelope (NE) and the cytoplasm and shows discontinuous, punctate staining along the rim of the NE. This antibody can inhibit import of nuclear localization sequence-containing proteins in permeabilized cells.
The MA3-070 antigen is purified NTF97 from bovine erythrocytes.
Target Information
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HGNC:6400; importin 1; importin 90; Importin beta; importin beta-1 subunit; Importin subunit beta-1; Importin-90; KapB1; karyopherin (importin) beta 1; karyopherin beta 1; Karyopherin subunit beta-1; karyopherin,beta 1; MGC2155; MGC2156; MGC2157; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97; SCG
Gene Aliases: AA409963; IMB1; Impnb; IPO1; IPOB; KPNB1; NTF97
UniProt ID: (Human) Q14974, (Rat) P52296, (Mouse) P70168
Entrez Gene ID: (Bovine) 507804, (Dog) 491042, (Pig) 100518449, (Human) 3837, (Rat) 24917, (Mouse) 16211
protein import into nucleus, docking
protein import into nucleus, translocation
apoptotic DNA fragmentation
protein import into nucleus
NLS-bearing protein import into nucleus
ribosomal protein import into nucleus
mitotic chromosome movement towards spindle pole
mitotic metaphase plate congression
modulation by virus of host process
astral microtubule organization
Ran protein signal transduction
establishment of mitotic spindle localization
establishment of protein localization
intracellular transport of virus
mitotic spindle assembly
intracellular protein transport
nucleocytoplasmic transport
transport
protein transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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