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Invitrogen
BrdU Monoclonal Antibody (BU-1)
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FIGURE: 1 / 14
BrdU Antibody (MA3-071) in ICC/IF
Immunofluorescent analysis of BrdU (green) showing staining in the nucleus of Hela cells treated with 100nM BrdU (right) and untreated Hela cells (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BrdU monoclonal antibody (Product # MA3-071) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary ... View More
Product Details
MA3-071
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical,
Chicken,
Mouse,
Non-human primate
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
BU-1
Immunogen
5-iodouridine covalently coupled to ovalbumin.
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Amount
5 µg
Purification
Protein A
Storage buffer
0.01M HEPES with 0.15M NaCl, 30% glycerol
Contains
0.01% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-071 detects 5-Bromo-2-deoxyuridine (BrdU) in many samples.
MA3-071 has been successfully used in immunofluorescence, immunohistochemistry, and flow cytometry applications.
The MA3-071 immunogen is 5-iodouridine covalently coupled to ovalbumin.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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