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Invitrogen
Lamin B2 Monoclonal Antibody (E-3), Alexa Fluor™ 555
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FIGURE: 1 / 1
Lamin B2 Antibody (MA3-32100-A555) in ICC/IF
Immunofluorescent analysis of Lamin B2 (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Lamin B2 Monoclonal Antibody, AlexaFluor 555 conjugate (Product # MA3-32100-A555) at a dilution of 1.25 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking ... View More
Product Details
MA3-32100-A555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
E-3
Immunogen
Extracts from chicken nuclei
Conjugate
Excitation/Emission Max
553/568 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: lamin A/C; lamin B2; lamin B3; Lamin-B2; MGC2721; unnamed protein product
Gene Aliases: EPM9; LAMB2; lamin-B2; LMN2; LMNA; LMNB2; RGD1563803
UniProt ID: (Human) Q03252, (Chicken) P14732, (Mouse) P21619
Entrez Gene ID: (Human) 84823, (Chicken) 396222, (Mouse) 16907, (Rat) 299625
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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