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Invitrogen
GST Tag Monoclonal Antibody (8-326), DyLight™ 800 4X PEG
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GST Tag Antibody (MA4-004-D800) in WB
Western blot was performed using GST Tag Monoclonal Antibody (8-326), DyLight 800 4X PEG (Product # MA4-004-D800) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), H3-GST, 50 µg (Lane 3), H3-GST, 25 µg (Lane 4), H3-GST, 12.5 µg (Lane 5), H3-GST, 6.25 µg (Lane 6), GST-p65, 50 µg (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulo... View More
Product Details
MA4-004-D800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
8-326
Immunogen
Recombinant purified full-length GST
Conjugate
Excitation/Emission Max
789/794 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
MES with proprietary stabilizer
Contains
no preservative
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA4-004-D800 detects GST tag from various samples. MA4-004-D800 has been successfully used for fluorescent detection of GST tagged proteins in Western Blotting.
DyLight 800 4X PEG has an excitation/emission of 783/797nm.
Target Information
GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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