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Invitrogen
Cytokeratin Pan Type II Monoclonal Antibody (AE3)
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FIGURE: 1 / 3
Cytokeratin Pan Type II Antibody (MA5-13150) in IHC (P)
Immunohistochemical analysis of Cytokeratin Pan Type II showing staining in the cytoplasm and membrane of epithelial cells on formalin-fixed paraffin-embedded human lung carcinoma tissue. The section was pre-treated using xylene as a clearing agent, followed by heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. Enzyme activity was blocked on the tissue using 3% hydrogen pero... View More
Product Details
MA5-13150
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
AE3
Immunogen
Human epidermal Keratin
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-13150 targets Cytokeratin High Molecular Weight in IF and IHC (P) applications and shows reactivity with Bovine, Chicken, Human, mouse, Non-human primate, Rabbit, and Rat samples.
The MA5-13150 immunogen is human epidermal Keratin.
This antibody detects basic (Type II) cytokeratins: Cytokeratin 1, Cytokeratin 2, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 6, Cytokeratin 7 and Cytokeratin 8.
Target Information
Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 58 kDa cytokeratin; 65 kDa cytokeratin; 67 kDa cytokeratin; CK-1; CK-2e; CK-2P; CK-3; CK-4; CK-5; CK-6A; CK-6D; CK-7; CK-72; CK-8; cytokeratin 1; cytokeratin 2; cytokeratin 3; cytokeratin 4; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; cytokeratin 7; cytokeratin 8; Cytokeratin-1; Cytokeratin-2e; Cytokeratin-2P; Cytokeratin-3; Cytokeratin-4; Cytokeratin-5; Cytokeratin-6A; Cytokeratin-6D; Cytokeratin-7; Cytokeratin-72; Cytokeratin-8; cytoskeletal protein; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; Epithelial keratin-2e; Hair alpha protein; intermediate filament protein; K7; K1; K2e; K2P; K3; K4; K5; K6A; K7; K72; K76; K8; keratin 1, type II; keratin 2, type II; keratin 2p; keratin 3, type II; keratin 4, type II; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6, inner root sheath, 2; keratin 6A, , type II; keratin 6A, type II; keratin 7, type II; keratin 72, type II; keratin 76, type II; keratin 8, type II; keratin K5; keratin protein K6irs; keratin type II; keratin, 55K type II cytoskeletal; keratin, epidermal type II, K6A; keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 2 epidermal; Keratin, type II cytoskeletal 2 oral; Keratin, type II cytoskeletal 3; Keratin, type II cytoskeletal 4; Keratin, type II cytoskeletal 5; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 7; Keratin, type II cytoskeletal 72; Keratin, type II cytoskeletal 8; Keratin-1; Keratin-2 epidermis; Keratin-2e; Keratin-3; Keratin-4; Keratin-5; Keratin-6A; Keratin-7; Keratin-72; Keratin-76; Keratin-8; KRT5; MGC116967; MGC116968; pan keratin; Sarcolectin; Type II inner root sheath-specific keratin-K6irs2; type II intermediate filament protein; type II keratin subunit protein; type II mesothelial keratin K7; Type-II keratin Kb1; Type-II keratin Kb2; Type-II keratin Kb3; Type-II keratin Kb35; Type-II keratin Kb4; Type-II keratin Kb5; Type-II keratin Kb6; Type-II keratin Kb7; Type-II keratin Kb8; Type-II keratin Kb9; unnamed protein product
Gene Aliases: AEI2; CARD2; CK-2e; CK-4; CK-6C; CK-6E; CK-72; CK-8; CK1; CK3; CK4; CK5; CK6A; CK6C; CK6D; CK7; CK8; CYK4; CYK8; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; EHK; EHK1; EPPK; HUMCYT2A; K1; K2C7; K2C8; K2e; K3; K4; K5; K6A; K6C; K6D; K6irs; K6IRS2; K7; K72; K8; KB35; KO; KRT1; KRT1A; KRT2; KRT2A; KRT2B; KRT2E; KRT2P; KRT3; KRT4; KRT5; KRT5A; KRT6; KRT6A; KRT6C; KRT6D; KRT6IRS2; KRT7; KRT72; KRT76; KRT8; KRTA; KRTE; MECD2; NEPPK; PC3; SCL; WSN1
UniProt ID: (Human) P04264, (Human) P35908, (Human) P12035, (Human) P19013, (Human) P13647, (Human) P02538, (Human) P08729, (Human) Q14CN4, (Human) Q01546, (Human) P05787
Entrez Gene ID: (Human) 3848, (Human) 3849, (Human) 3850, (Human) 3851, (Human) 3852, (Human) 3853, (Human) 3855, (Human) 140807, (Human) 51350, (Human) 3856
cytoskeleton organization
epithelial cell differentiation
keratinization
intermediate filament organization
negative regulation of epithelial cell proliferation
morphogenesis of an epithelium
positive regulation of cell proliferation
cell differentiation
killing of cells of other organism
wound healing
defense response to Gram-positive bacterium
antimicrobial humoral immune response mediated by antimicrobial peptide
negative regulation of entry of bacterium into host cell
tumor necrosis factor-mediated signaling pathway
response to other organism
cell differentiation involved in embryonic placenta development
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
epidermis development
pigmentation
sebaceous gland development
complement activation, lectin pathway
response to oxidative stress
peptide cross-linking
fibrinolysis
regulation of angiogenesis
negative regulation of inflammatory response
protein heterotetramerization
establishment of skin barrier
keratinocyte development
keratinocyte activation
keratinocyte proliferation
positive regulation of epidermis development
keratinocyte migration
intermediate filament cytoskeleton organization
response to mechanical stimulus
regulation of cell migration
regulation of protein localization
intermediate filament polymerization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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