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Invitrogen
TLR7 Monoclonal Antibody (4G6), PE
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FIGURE: 1 / 2
TLR7 Antibody (MA5-16249) in Flow
Flow cytometry analysis of TLR7 in THP-1 cells. Samples were incubated with TLR7 monoclonal antibody (Product # MA5-16249) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. An intracellular stain was performed with primary antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.
Product Details
MA5-16249
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
4G6
Immunogen
A partial human TLR7 recombinant protein (amino acids 562-839).
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.50 mg/mL
Amount
50 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
TLR7 is a 10 amino acid long highly conserved membrane protein belonging to the Toll-like receptor (TLR) family with 27 LRR (leucine-rich) repeats and a TIR domain. TLR7 plays a fundamental role in pathogen recognition and activation of innate immunity. TLR7 is activated by infections with single-stranded RNA viruses, including influenza virus and vesicular stomatitis virus (VSV). TLR7 is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8. TLR7 also acts via MyD88 and TRAF6, leading to NF-kappaB activation, cytokine secretion and the inflammatory response. TLR7 recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RP23-139P21.3; Toll-like receptor 7; toll-like receptor 7-like; unnamed protein product
Gene Aliases: IMD74; SLEB17; TLR7; TLR7-like; UNQ248/PRO285
UniProt ID: (Human) Q9NYK1, (Mouse) P58681
Entrez Gene ID: (Human) 51284, (Mouse) 170743
MAPK cascade
toll-like receptor signaling pathway
immune system process
inflammatory response
immune response
signal transduction
I-kappaB kinase/NF-kappaB signaling
JNK cascade
positive regulation of chemokine production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interferon-gamma production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
toll-like receptor 7 signaling pathway
toll-like receptor 8 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of transcription from RNA polymerase II promoter
positive regulation of inflammatory response
defense response to virus
positive regulation of macrophage cytokine production
response to cGMP
cellular response to mechanical stimulus
defense response to other organism
cellular response to virus
endolysosomal toll-like receptor signaling pathway
microglial cell activation
regulation of protein phosphorylation
MyD88-dependent toll-like receptor signaling pathway
I-kappaB phosphorylation
positive regulation of NF-kappaB import into nucleus
positive regulation of interferon-gamma biosynthetic process
positive regulation of interferon-alpha biosynthetic process
positive regulation of interferon-beta biosynthetic process
positive regulation of interleukin-8 biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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