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Invitrogen
beta Tubulin Loading Control Monoclonal Antibody (BT7R)
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FIGURE: 1 / 36
beta Tubulin Loading Control Antibody (MA5-16308) in ICC/IF
Immunofluorescent analysis of Beta-Tubulin (red) in HEK293T cells. Cells fixed in 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a Beta-Tubulin monoclonal antibody (Product # MA5-16308) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and in... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-16308
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
Published species
Avian,
C. elegans,
Chicken,
Fruit fly,
Hamster,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat,
Sheep
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
BT7R
Immunogen
KLH conjugated peptide of the N-terminus of Beta-Tubulin.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta 5-tubulin; beta tubulin; beta tubulin 1; beta-4 tubulin; beta-4-tubulin; beta-5 tubulin; beta-tubulin; beta-tubulin 1; beta-tubulin 2; beta-tubulin 3; beta-tubulin 4; beta-tubulin 5; beta-tubulin 6; beta-tubulin 7; beta-tubulin 8; beta-tubulin 9; Beta-tubulin class-I; Beta-tubulin class-II; beta-tubulin class-III; Beta-tubulin class-V; Beta-tubulin class-VI; beta-tubulin T beta15 (aa 1-445); betaIII-tubulin; c(beta)7 tubulin; class I beta-tubulin; class IIa beta-tubulin; class III beta-tubulin; class IVa beta-tubulin; dJ40E16.7; M40 antibody; Neuron-specific class III beta-tubulin; T beta-15; TUB; TUB1; TUB2; TUB3; TUB4; TUB5; TUB6; TUB7; TUB8; TUB9; TUBB; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; tubulin beta; Tubulin beta 4'; tubulin beta chain; Tubulin beta class IIa; Tubulin beta-1 chain; Tubulin beta-2 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-7 chain; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIa; tubulin, beta 3; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta polypeptide; tubulin, beta polypeptide 2; unnamed protein product
Gene Aliases: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; BB220206; brdp; CDCBM5; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; RGD1305887; RGD1309427; TUBB; Tubb1; TUBB2; TUBB2A; Tubb2b; Tubb3; TUBB4; Tubb4a; Tubb5; Tubb6
UniProt ID: (Chicken) P09244, (Chicken) P09207, (Chicken) P32882, (Chicken) P09653, (Mouse) A2AQ07, (Rat) P85108, (Mouse) Q7TMM9, (Mouse) Q9CWF2, (Rat) Q3KRE8, (Mouse) Q9ERD7, (Rat) Q4QRB4, (Mouse) Q9D6F9, (Mouse) P99024, (Rat) P69897, (Mouse) Q922F4
Entrez Gene ID: (Rabbit) 100349792, (Chicken) 396254, (Dog) 474830, (Rabbit) 100358689, (Chicken) 396427, (Rabbit) 100347858, (Dog) 485950, (Human) 7280, (Chicken) 768337, (Dog) 478702, (Chicken) 420883, (Chicken) 431043, (Dog) 102154194, (Dog) 100855815, (Rabbit) 100348290, (Rabbit) 100350252, (Chicken) 421037, (Dog) 480213, (Mouse) 545486, (Rat) 679312, (Rat) 498736, (Mouse) 22151, (Mouse) 73710, (Rat) 291081, (Mouse) 22152, (Rat) 246118, (Rat) 29213, (Mouse) 22153, (Mouse) 22154, (Rat) 29214, (Mouse) 67951, (Rat) 307351
microtubule cytoskeleton organization
mitotic cell cycle
microtubule-based process
regulation of synapse organization
spindle assembly
neuron migration
cytoskeleton organization
cerebral cortex development
negative regulation of microtubule polymerization
axon guidance
neuron differentiation
protein polymerization
metabolic process
mitotic nuclear division
cell projection organization
cellular process
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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