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Cytokeratin 19 Monoclonal Antibody (A53-B/A2), Alexa Fluor™ 488
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FIGURE: 1 / 2
Cytokeratin 19 Antibody (MA5-18158) in Flow
Flow Cytometry analysis of Cytokeratin 19 using Cytokeratin 19 Monoclonal Antibody (A53-B/A2), Alexa Fluor™ 488 (Product # MA5-18158). Separation of human MCF-7 cells (red-filled) from leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 Alexa Fluor™ 488 antibody (concentration in sample 5 μg/mL).
Product Details
MA5-18158
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
A53-B/A2
Immunogen
MCF-7 human breast adenocarcinoma cell line
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Size-exclusion chromatography
Storage buffer
PBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
Target Information
Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: 40-kDa keratin intermediate filament; C K1CS; CK 19; CK-19; cytokeratin 19; Cytokeratin-19; K19; keratin 19, type I; Keratin, type I cytoskeletal 19; keratin, type I, 40-kd; Keratin-19; MGC15366; unnamed protein product
Gene Aliases: CK19; K19; K1CS; KRT19
UniProt ID: (Human) P08727
Entrez Gene ID: (Human) 3880
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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