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Invitrogen
METTL3 Monoclonal Antibody (OTI1B7)
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FIGURE: 1 / 4
METTL3 Antibody (MA5-27527) in WB, ELISA, IP
PLoS pathogens 2021 -
Fig 3 DDX5 regulated the m6A writer complex by recruiting METTL3 after VSV infection. (A, B): VSV promoted the interaction of DDX5 and METTL3 in MEFs. MEFs were infected with VSV or treated with Poly (I:C) for 8h and lysed with NP40 lysis buffer. Lysates were subjected to IP with DDX5 antibody or IgG. Whole-cell and IP lysates were analyzed by western blotting. The DDX5-METTL3 interaction complex was quanti... View More
Product Details
MA5-27527
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1B7
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-284 of METTL3 produced in E.coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
METTL3 is the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; hMETTL3; m6a methyltransferase; methyltransferase like 3; methyltransferase-like 3; Methyltransferase-like protein 3; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; MT-A70; N(6)-adenosine-methyltransferase 70 kDa subunit; N(6)-adenosine-methyltransferase catalytic subunit METTL3; N6-adenosine-methyltransferase 70 kDa subunit; N6-adenosine-methyltransferase subunit METTL3; unnamed protein product
Gene Aliases: 2310024F18Rik; hMETTL3; IME4; M6A; METTL3; MT-A70; MTA70; Spo8
UniProt ID: (Human) Q86U44, (Mouse) Q8C3P7
Entrez Gene ID: (Human) 56339, (Mouse) 56335, (Rat) 361035
mRNA (N6-adenosine)-methyltransferase activity
RNA binding
mRNA binding
protein binding
methyltransferase activity
RNA methyltransferase activity
transferase activity
protein heterodimerization activity
S-adenosyl-L-methionine binding
mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity
RNA metabolism protein
RNA methyltransferase
nucleic acid metabolism protein
mRNA splicing, via spliceosome
RNA methylation
immune system process
mRNA processing
mRNA export from nucleus
cellular response to DNA damage stimulus
spermatogenesis
circadian rhythm
dosage compensation by inactivation of X chromosome
mRNA modification
stem cell population maintenance
forebrain radial glial cell differentiation
cell differentiation
primary miRNA processing
methylation
cellular response to UV
gliogenesis
innate immune response
regulation of T cell differentiation
positive regulation of translation
negative regulation of Notch signaling pathway
oogenesis
rhythmic process
regulation of meiotic cell cycle
negative regulation of type I interferon-mediated signaling pathway
mRNA destabilization
endothelial to hematopoietic transition
regulation of hematopoietic stem cell differentiation
positive regulation of cap-independent translational initiation
nucleobase-containing compound metabolic process
adenosine to inosine editing
mRNA methylation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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