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Invitrogen
NALCN Monoclonal Antibody (N187/7)
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FIGURE: 1 / 2
NALCN Antibody (MA5-27593) in ICC/IF
Immunofluorescent analysis of NALCN in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with NALCN monoclonal antibody (Product # MA5-27593) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) NALCN Antibody, D) Merged image. Magnification... View More
Product Details
MA5-27593
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N187/7
Immunogen
Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27593 was sufficient for detection of NALCN in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 200kDa.
This antibody was formerly sold as clone S187-7.
Target Information
Nalcn is a voltage-independent, cation-nonselective channel which is permeable to sodium, potassium and calcium ions. Nalcn regulates the resting membrane potential and controls neuronal excitability (PubMed:17448995). Neuropeptides such as neurotensin and substance P (SP) stimulate the firing of action potentials by activating NALCN through a SRC family kinases-dependent pathway. In addition to its baseline activity, Nalcn activity is enhanced/modulated by several GPCRs, is required for normal respiratory rhythm and neonatal survival, is involved in systemic osmoregulation by controlling the serum sodium concentration. Nalcn is partly responsible for the substance P-induced depolarization and regulation of the intestinal pace-making activity in the interstitial cells of Cajal. Further, Nalcn plays a critical role in both maintenance of spontaneous firing of substantia nigra pars reticulata (SNr) neurons and physiological modulation of SNr neuron excitability. Diseases associated with NALCN include Congenital contractures of the limbs and face, Hypotonia, Developmental Delay and Hypotonia, Infantile Psychomotor Retardation And Characteristic Facies 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain voltage-gated cation channel; CanIon; FLJ23913; FLJ44659; FLJ44764; four domain-type voltage-gated ion channel alpha-1 subunit; four repeat voltage-gated ion channel; MGC74524; Sodium leak channel NALCN; Sodium leak channel non-selective protein; unnamed protein product; voltage gated channel like 1; Voltage gated channel-like protein 1
Gene Aliases: A530023G15Rik; AI849508; bA430M15.1; CanIon; CLIFAHDD; IHPRF; IHPRF1; INNFD; NALCN; VGCNL1
UniProt ID: (Human) Q8IZF0, (Mouse) Q8BXR5
Entrez Gene ID: (Human) 259232, (Mouse) 338370, (Rat) 266760
ion transport
sodium ion transport
positive regulation of synaptic transmission, cholinergic
positive regulation of synaptic transmission, GABAergic
ion transmembrane transport
sodium ion transmembrane transport
transmembrane transport
regulation of resting membrane potential
calcium ion transmembrane transport
potassium ion transmembrane transport
transport
regulation of ion transmembrane transport
calcium ion import
membrane depolarization during action potential
sperm motility
cation transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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