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Invitrogen
TRPV3 Monoclonal Antibody (N15/39)
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FIGURE: 1 / 3
TRPV3 Antibody (MA5-27597) in ICC/IF
Immunofluorescent analysis of TRPV3 in human HaCaT cells. Sample was fixed using 100% cold methanol (10 minutes at -20°C), incubated with TRPV3 monoclonal antibody (Product # MA5-27597) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27597
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N15/39
Immunogen
Synthetic peptide amino acids 458-474 (cytoplasmic C-terminus) of rat TrpV3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27597 was sufficient for detection of TrpV3 in 10 µg of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody|Detects approximately 70kDa.
Target Information
TRPV3 belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. This gene lies in close proximity to another family member (TRPV1) gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: heat sensitive channel TRPV3; ion channel; MGC124324; MGC124325; non-hair; TRP ion channel TRPV3; unnamed protein product; vanilloid receptor-like 3; vanilloid receptor-related osmotically activated channel protein; VRL 3; VRL-3
Gene Aliases: 1110036I10Rik; FNEPPK2; Nh; OLMS; OLMS1; VRL3
UniProt ID: (Human) Q8NET8, (Mouse) Q8K424
Entrez Gene ID: (Human) 162514, (Rat) 497948, (Mouse) 246788
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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