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Invitrogen
CD55 Recombinant Rabbit Monoclonal Antibody (076)
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FIGURE: 1 / 2
CD55 Antibody (MA5-29678) in ICC/IF
Immunofluorescence staining of mouse CD55 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD55 Recombinant Rabbit Monoclonal Antibody (76) (Product # MA5-29678, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-29678
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
076
Immunogen
Recombinant Mouse CD55/DAF Protein (Met1-Thr361)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Mouse CD55/DAF.
Target Information
CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can regulate complement when bound to CD97. In tumors, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis. CD55 is a 70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells, the apparent molecular weight is somewhat larger for CD55 and has a substantial content of O-glycans. CD55 binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. CD55 carries the Cromer related blood group antigens. Two alternatively spliced transcripts encoding CD55 have been identified, and the predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. CD55 has been studied to have a role in prostate cancer growth and survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD55 antigen; complement-glycosylphosphatidylinositol; Cromer blood group; Daf1; decay accelerating factor 1; glycosylphoshatidylinositol-anchored form; GPI anchor addition signal; RP11-357P18.1; secreted form; unnamed protein product
Gene Aliases: Daf; Daf-GPI; Daf1; GPI-DAF
UniProt ID: (Mouse) Q61475
Entrez Gene ID: (Mouse) 13136
regulation of lymphocyte mediated immunity
positive regulation of T cell cytokine production
regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains
positive regulation of cytosolic calcium ion concentration
regulation of complement activation
regulation of complement activation, classical pathway
regulation of lipopolysaccharide-mediated signaling pathway
negative regulation of complement activation
viral entry into host cell
regulation of complement-dependent cytotoxicity
positive regulation of CD4-positive, alpha-beta T cell activation
positive regulation of CD4-positive, alpha-beta T cell proliferation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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