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Invitrogen
alpha Tubulin Monoclonal Antibody (GT114), HRP
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FIGURE: 1 / 1
alpha Tubulin Antibody (MA5-31467) in WB
alpha Tubulin Monoclonal Antibody (GT114), HRP detects alpha Tubulin protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract. C. 30 µg NCI-H929 whole cell lysate/extract.10 % SDS-PAGE. Alpha Tubulin Monoclonal Antibody (GT114), HRP (Product # MA5-31467) dilution: 1:10,000.
Product Details
MA5-31467
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
GT114
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Protein G
Storage buffer
TBS, pH 7.4, with 1% BSA
Contains
0.005% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Positive Control: Jurkat, Raji, NCI-H929.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha tubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; Alpha-tubulin 6; Alpha-tubulin ubiquitous; class II beta-tubulin isotype; class IIb beta-tubulin; epididymis secretory sperm binding protein; FLJ30169; gangliocyte protein related to virus stimulation; H2 ALPHA; hum-a-tub1; hum-a-tub2; MGC8685; similar to beta tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; Tubulin alpha chain-like 3; tubulin alpha-1; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1 (testis specific); tubulin, alpha 2; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, beta 2B; tubulin, beta polypeptide paralog; unnamed protein product
Gene Aliases: ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; CDCBM7; CMYO26; FTDALS9; H2-ALPHA; K-ALPHA-1; LIS3; OZEMA23; OZEMA24; PMGYSA; SPAX11; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; TUBA4A; TUBA6; TUBAL3; TUBB2B
UniProt ID: (Human) Q71U36, (Human) P68363, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) Q9BVA1
Entrez Gene ID: (Human) 7846, (Human) 10376, (Human) 84790, (Human) 7278, (Human) 112714, (Human) 7277, (Human) 79861, (Human) 347733
nucleotide binding
structural molecule activity
structural constituent of cytoskeleton
protein binding
GTP binding
hydrolase activity
identical protein binding
macromolecular complex binding
metal ion binding
protein heterodimerization activity
double-stranded RNA binding
GTPase activity
ubiquitin protein ligase binding
enzyme binding
protein kinase binding
microtubule cytoskeleton organization
mitotic cell cycle
microtubule-based process
cytoskeleton-dependent intracellular transport
cell division
cellular response to interleukin-4
cytoskeleton organization
neuron migration
nervous system development
cerebral cortex development
modulation of synaptic transmission
positive regulation of axon guidance
embryonic brain development
startle response
intracellular protein transport
centrosome cycle
smoothened signaling pathway
memory
locomotory behavior
adult locomotory behavior
visual learning
response to mechanical stimulus
glial cell differentiation
gene expression
dentate gyrus development
cerebellar cortex morphogenesis
hippocampus development
pyramidal neuron differentiation
neurogenesis
neuron differentiation
sperm motility
adult behavior
response to tumor necrosis factor
locomotory exploration behavior
microtubule polymerization
forebrain morphogenesis
homeostasis of number of cells within a tissue
regulation of synapse organization
synapse organization
neuron apoptotic process
motor behavior
cellular response to calcium ion
organelle transport along microtubule
neuron projection arborization
response to L-glutamate
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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