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Invitrogen
Phospho-MSK1 (Thr581) Recombinant Rabbit Monoclonal Antibody (MSK1T581-A5)
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FIGURE: 1 / 6
Phospho-MSK1 (Thr581) Antibody (MA5-37014) in WB
Western blot analysis of Phospho-MSK1 (Thr581) using 293T cell extract. Samples were untreated or treated with staurosporine, and incubated in monoclonal Phospho-MSK1 (Thr581) antibody (Product # MA5-37014) with a dilution of 0.02 µg/mL.
Product Details
MA5-37014
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
MSK1T581-A5
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Thr581 of human phospho MSK1.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
10 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
RPS6KA5 (MSK1) is a serine/threonine protein kinase that is essential for the regulation of various cellular processes. RPS6KA5 is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 90 kDa ribosomal protein S6 kinase 5; kinase MSK1; KS6A5; mitogen and stress-activated protein kinase 1; Nuclear mitogen- and stress-activated protein kinase 1; Ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kDa, polypeptide 5; RLSK; RSK-like protein kinase; RSKL; S6K-alpha-5; S6PKH2; unnamed protein product
Gene Aliases: 3110005L17Rik; 6330404E13Rik; AI854034; MSK1; MSPK1; RLPK; RPS6KA5
UniProt ID: (Human) O75582, (Mouse) Q8C050
Entrez Gene ID: (Human) 9252, (Mouse) 73086, (Rat) 314384
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
histone kinase activity (H3-S10 specific)
histone kinase activity (H3-S28 specific)
histone H2AS1 kinase activity
metal ion binding
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
negative regulation of cytokine production
chromatin remodeling
regulation of transcription, DNA-templated
protein phosphorylation
inflammatory response
axon guidance
positive regulation of CREB transcription factor activity
intracellular signal transduction
TORC1 signaling
post-translational protein modification
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of NF-kappaB transcription factor activity
interleukin-1-mediated signaling pathway
regulation of postsynapse organization
phosphorylation
histone phosphorylation
positive regulation of histone phosphorylation
positive regulation of histone acetylation
histone H3-S10 phosphorylation
histone H3-S28 phosphorylation
histone H2A-S1 phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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