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Invitrogen
Phospho-NFKB p65 (Ser536) Recombinant Rabbit Monoclonal Antibody (NFKBp65S536-B7), PE
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FIGURE: 1 / 1
Phospho-NFKB p65 (Ser536) Antibody (MA5-37403) in Flow
Flow cytometry of Phospho-NFKB p65 (Ser536) using HeLa cells. Samples were incubated in monoclonal Phospho-NFKB p65 (Ser536) antibody (Product # MA5-37403). Images correspond with the following conditions: untreated (red), treated with TNFa plus CalA (green).
Product Details
MA5-37403
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
NFKBp65S536-B7
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser536 of human phospho-NFKB p65.
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Protein A/G
Storage buffer
PBS with 0.20% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EBP-1; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kB p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; REL A; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A
Gene Aliases: NFKB3; p65; RELA
UniProt ID: (Human) Q04206
Entrez Gene ID: (Human) 5970
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
transcription factor binding
protein kinase binding
chromatin DNA binding
ubiquitin protein ligase binding
protein complex binding
activating transcription factor binding
phosphate ion binding
identical protein binding
protein homodimerization activity
actinin binding
histone deacetylase binding
transcription regulatory region DNA binding
protein heterodimerization activity
protein N-terminus binding
NF-kappaB binding
repressing transcription factor binding
ankyrin repeat binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
liver development
hair follicle development
stimulatory C-type lectin receptor signaling pathway
acetaldehyde metabolic process
transcription from RNA polymerase II promoter
inflammatory response
cellular defense response
aging
positive regulation of cell proliferation
organ morphogenesis
response to organic substance
response to UV-B
positive regulation of Schwann cell differentiation
cytokine-mediated signaling pathway
membrane protein intracellular domain proteolysis
positive regulation of chondrocyte differentiation
positive regulation of type I interferon production
response to muramyl dipeptide
response to progesterone
response to insulin
response to cobalamin
cellular response to hepatocyte growth factor stimulus
response to muscle stretch
Fc-epsilon receptor signaling pathway
negative regulation of protein catabolic process
response to drug
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to amino acid
response to morphine
positive regulation of interleukin-12 biosynthetic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin receptor signaling pathway
regulation of inflammatory response
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
response to cAMP
defense response to virus
cellular response to hydrogen peroxide
nucleotide-binding oligomerization domain containing 2 signaling pathway
response to interleukin-1
cellular response to lipopolysaccharide
cellular response to nicotine
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to peptide hormone stimulus
negative regulation of NIK/NF-kappaB signaling
positive regulation of miRNA metabolic process
negative regulation of extrinsic apoptotic signaling pathway
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