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Invitrogen
CD16 Monoclonal Antibody (3G8), FITC
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FIGURE: 1 / 7
CD16 Antibody (MA5-44096) in Flow
Separation of human CD16 positive CD3 negative lymphocytes (red-filled) from CD16 negative CD3 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD16 (3G8) FITC Monoclonal antibody (Product # MA5-44096) using a dilution of 4 µL reagent/100 µL of peripheral whole blood.
Product Details
MA5-44096
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
3G8
Immunogen
Human neutrophils
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects both 158V and 158F allotype on natural NK cells.
Target Information
CD16, known as Fc gamma receptor III, exists in two distinct isoforms: Fc gamma RIIIA and Fc gamma RIIIB. These isoforms are encoded by the genes FCGR3A and FCGR3B, respectively, which are located on chromosome 1 and share a high degree of similarity. Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as natural killer (NK) cells, but not on T or B cells. This receptor recognizes the Fc portion of immunoglobulin G (IgG) and is involved in the clearance of immune complexes from circulation. Upon binding IgG, Fc gamma RIIIA activates signal transduction pathways that result in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation. Fc gamma RIIIB is expressed on polymorphonuclear neutrophils (PMN) as a glycosylphosphatidylinositol (GPI)-anchored form. It plays a role in similar immune functions, including cellular-mediated cytotoxicity and enhancement of virus infections. Mutations in the FCGR3A gene have been associated with Immunodeficiency 20 and linked to susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; Fc fragment of IgG low affinity IIIa receptor (CD16a) isoform FCGR3A_1; Fc fragment of IgG low affinity IIIa receptor (CD16a) isoform FCGR3A_2; Fc fragment of IgG receptor IIIa; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor III-B; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; fc-gamma RIIIb; Fc-gamma-RIIb; Fc-gamma-RIIc; FCG2; FcgammaRIIIA; FcgammaRIIIb; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; IgG receptor precursor; immunoglobulin G Fc receptor III; low affinity IgG receptor; low affinity IgG receptor CD16; low affinity IgG receptor precursor; Low affinity immunoglobulin gamma Fc region receptor III-A; low affinity immunoglobulin gamma Fc region receptor III-A/III-B recombinant; Low affinity immunoglobulin gamma Fc region receptor III-B; low affinity immunoglobulin gamma receptor III-a Fc fragment; neutrophil-specific antigen NA; RP11-5K23.1; unnamed protein product
Gene Aliases: CD16; CD16-I; CD16-II; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FcGRIIIA; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20
UniProt ID: (Human) P08637, (Human) O75015
Entrez Gene ID: (Human) 2214, (Human) 2215
antibody-dependent cellular cytotoxicity
immune response
cell surface receptor signaling pathway
Fc-gamma receptor signaling pathway
T cell mediated cytotoxicity
immune system process
protein import
calcium-mediated signaling
natural killer cell activation
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell proliferation
macrophage activation
natural killer cell mediated cytotoxicity
natural killer cell degranulation
protein kinase B signaling
protein transmembrane transport
granzyme-mediated programmed cell death signaling pathway
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