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Invitrogen
HSP27 Monoclonal Antibody (8A7), FITC
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FIGURE: 1 / 4
HSP27 Antibody (MA5-45122) in IHC (P)
Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Hsp27 monoclonal antibody (Product # MA5-45122) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Product Details
MA5-45122
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Bovine,
Dog,
Guinea pig,
Mouse,
Sheep,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
8A7
Immunogen
HSP27 peptide
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:5000 dilution of MA5-45122 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.|Detects approximately 25kDa or approximately 27kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
Target Information
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 27 kDa heat shock protein 1; 27-kda heat shock protein; 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27 kDa protein 1; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; heat shock protein 27 kDa beta-1; Heat shock protein beta-1; Heat shock protein family B member 1; heat shock protein family B member 1 variant 1; heat shock protein family B member 1 variant 2; heat shock protein, 25 kDa; HS; HSP; HSP 25; HSP 27; HSP27; HSPB1; p25; Phospho-HSP 27; SRP27; Stress-responsive protein 27; truncated hsp25; unnamed protein product
Gene Aliases: 27kDa; CMT2F; HEL-S-102; HMN2B; HMND3; HS.76067; Hsp25; HSP27; HSP28; HSPB1; I79_007264; SRP27
UniProt ID: (Human) P04792, (Bovine) Q3T149, (Mouse) P14602
RNA binding
protein kinase C binding
protein binding
protein kinase C inhibitor activity
protein kinase binding
identical protein binding
protein homodimerization activity
ubiquitin binding
protein binding involved in protein folding
unfolded protein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
poly(A) RNA binding
regulation of protein phosphorylation
regulation of translational initiation
protein folding
response to unfolded protein
response to heat
response to virus
regulation of autophagy
positive regulation of interleukin-1 beta production
positive regulation of tumor necrosis factor production
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
protein refolding
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
vascular endothelial growth factor receptor signaling pathway
platelet aggregation
negative regulation of protein kinase C signaling
anterograde axonal protein transport
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of endothelial cell chemotaxis
negative regulation of apoptotic signaling pathway
chaperone-mediated protein folding
negative regulation of protein kinase activity
positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway
positive regulation of tumor necrosis factor biosynthetic process
negative regulation of protein serine/threonine kinase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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