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Invitrogen
HSP27 Monoclonal Antibody (5D12-A12), FITC
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FIGURE: 1 / 4
HSP27 Antibody (MA5-45206) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with Hsp27 monoclonal antibody (Product # MA5-45206) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp27 antibody. (C) Composite. Heat Shocked at 4... View More
Product Details
MA5-45206
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
5D12-A12
Immunogen
Full length human HSP27
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.2, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
0.5 µg/mL of MA5-45206 was sufficient for detection of HSP27 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 27kDa. Has no cross-reactivity to Alpha B crystallin. Very limited cross-reactivity to other species.
Target Information
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 27-kda heat shock protein; 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; Heat shock protein family B member 1; HS; HSP; HSP 27; HSP27; HSPB1; Phospho-HSP 27; SRP27; Stress-responsive protein 27; unnamed protein product
Gene Aliases: CMT2F; HEL-S-102; HMN2B; HMND3; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27
UniProt ID: (Human) P04792
Entrez Gene ID: (Human) 3315
RNA binding
protein kinase C binding
protein binding
protein kinase C inhibitor activity
protein kinase binding
identical protein binding
protein homodimerization activity
ubiquitin binding
protein binding involved in protein folding
unfolded protein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
regulation of protein phosphorylation
regulation of translational initiation
protein folding
response to unfolded protein
response to heat
response to virus
regulation of autophagy
positive regulation of interleukin-1 beta production
positive regulation of tumor necrosis factor production
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
protein refolding
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
vascular endothelial growth factor receptor signaling pathway
platelet aggregation
negative regulation of protein kinase C signaling
anterograde axonal protein transport
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of endothelial cell chemotaxis
negative regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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