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Invitrogen
HDEL Monoclonal Antibody (2E7), FITC
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Product Details
MA5-45241
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Plant,
Yeast
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2E7
Immunogen
Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
9.09mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45241 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 78kDa.
Target Information
HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains. GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CG5183-PA; CG5183-PB; CG5183-PC; ER lumen protein retaining receptor 2; ER lumen protein-retaining receptor; KDEL-receptor; KdelR-PA; KdelR-PB; KdelR-PC; KDELR1; lethal (2) 31Em
Gene Aliases: BEST:CK00230; CG5183; CK00230; Dmel\CG5183; Dmel_CG5183; dmErd2; ERD2; KdelR; l(2)31Em; l(2)k00311
UniProt ID: (Fruit fly) O76767
Entrez Gene ID: (Fruit fly) 34427
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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