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Invitrogen
VGLUT3 Monoclonal Antibody (N34/34), PerCP
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FIGURE: 1 / 2
VGLUT3 Antibody (MA5-45516) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with VGlut3 monoclonal antibody (Product # MA5-45516) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Syn... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45516
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N34/34
Immunogen
Fusion protein amino acids 546-588 (cytoplasmic C-terminus) of rat VGLUT3
Conjugate
PerCP
PerCP
PerCP
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45516 was sufficient for detection of VGLUT3 in 20 µg of CV-1 fibroblast cells (lysate) transfected with VGLUT3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 65kDa.
This antibody was formerly sold as clone S34-34.
Target Information
Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT-3 staining is also detectable in human cancers including those of the ovary, lung, and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: rVGLUT3; synaptic vesicle glutamate transporter; third isoform; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8; solute carrier family 17 (vesicular glutamate transporter), member 8; solute carrier family 17 member 8; unnamed protein product; vesicular neurotransmitter transporter; VGLUT 3
Gene Aliases: DFNA25; VGLUT3; Vgt3
UniProt ID: (Human) Q8NDX2, (Mouse) Q8BFU8, (Rat) Q7TSF2
Entrez Gene ID: (Human) 246213, (Mouse) 216227, (Rat) 266767
neural retina development
L-glutamate transport
synaptic transmission, glutamatergic
sodium ion transmembrane transport
sodium-dependent phosphate transport
regulation of synapse structure or activity
regulation of acetylcholine uptake
L-glutamate import
positive regulation of glutamate uptake involved in transmission of nerve impulse
phosphate ion homeostasis
transmembrane transport
cochlea development
neurotransmitter loading into synaptic vesicle
chloride transmembrane transport
neurotransmitter transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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