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          • Primary Antibodies ›
          • VGLUT3 Antibodies

          Invitrogen

          VGLUT3 Monoclonal Antibody (N34/34), PerCP

          View all (16) VGLUT3 antibodies

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          Cite VGLUT3 Monoclonal Antibody (N34/34), PerCP

          Additional Information:
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          • Antibody Testing Data (2)
          VGLUT3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          VGLUT3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          VGLUT3 Antibody (MA5-45516) in ICC/IF

          Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with VGlut3 monoclonal antibody (Product # MA5-45516) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Syn... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          VGLUT3 Antibody in Immunocytochemistry (ICC/IF)
          VGLUT3 Antibody in Immunocytochemistry (ICC/IF)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          VGLUT3 Monoclonal Antibody (N34/34), PerCP

          Product Details

          MA5-45516

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunohistochemistry (IHC)

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          N34/34

          Immunogen

          Fusion protein amino acids 546-588 (cytoplasmic C-terminus) of rat VGLUT3
          3D Epitope / Immunogen

          Conjugate

          PerCP PerCP PerCP
          • Unconjugated
          • APC 
          • FITC
          • PE 
          • Request custom conjugation

          Excitation/Emission Max

          482/675 nm View spectra spectra

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein G

          Storage buffer

          95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2931970

          Product Specific Information

          1 µg/mL of MA5-45516 was sufficient for detection of VGLUT3 in 20 µg of CV-1 fibroblast cells (lysate) transfected with VGLUT3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 65kDa.

          This antibody was formerly sold as clone S34-34.

          Target Information

          Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT-3 staining is also detectable in human cancers including those of the ovary, lung, and testis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8; Solute carrier family 17 member 8; Vesicular glutamate transporter 3; vesicular glutamate transporter-3; VGLUT 3; VGluT3

          View more View less

          Gene Aliases: BC042593; SLC17A8; VGLUT3

          View more View less

          UniProt ID: (Mouse) Q8BFU8, (Rat) Q7TSF2

          View more View less

          Entrez Gene ID: (Mouse) 216227, (Rat) 266767

          View more View less

          Function(s)
          L-glutamate transmembrane transporter activity symporter activity secondary carrier transporter
          Process(es)
          transport ion transport sodium ion transport neurotransmitter transport sensory perception of sound L-glutamate transport transmembrane transport L-glutamate transmembrane transport
          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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