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Invitrogen
GABBR1 Monoclonal Antibody (N93A/49), PE
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FIGURE: 1 / 3
GABBR1 Antibody (MA5-45539) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with GABA-B Receptor 1 monoclonal antibody (Product # MA5-45539) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red... View More
Product Details
MA5-45539
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N93A/49
Immunogen
Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45539 was sufficient for detection of GABA(B)R1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 115kDa. No cross-reactivity against GABA(B)R2.
This antibody was formerly sold as clone S93A-49.
Target Information
Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bM573K1.1.1 (gamma-aminobutyric acid (GABA) B receptor, 1a); DAAP-188P13.3; dJ271M21.1.1; dJ271M21.1.2; FLJ92613; G-protein coupled receptor; GABA(B) receptor; GABA-B receptor 1; GABA-B receptor, R1 subunit; GABA-B-R1; Gaba-b-r1a; Gaba-b1; GABA-BR1; Gaba-br1a receptor; GABA-BR1b receptor; GABAB; GABABR1; Gababr1 receptor; gamma-aminobutyric acid; gamma-aminobutyric acid (GABA) B receptor, 1; gamma-aminobutyric acid (GABA-B) receptor, 1; Gamma-aminobutyric acid type B receptor subunit 1; gb1; GBR1; HGB1a; metabotropic GABA receptor; OTTHUMP00000109099; OTTHUMP00000214725; seven transmembrane helix receptor; unnamed protein product
Gene Aliases: bM573K1.1; GABAB1; GABABR1; GABBR1; GABBR1-3; GB1; GPRC3A; NEDLC
UniProt ID: (Human) Q9UBS5, (Mouse) Q9WV18, (Rat) Q9Z0U4
Entrez Gene ID: (Human) 2550, (Mouse) 54393, (Rat) 81657
G-protein coupled receptor activity
G-protein coupled GABA receptor activity
protein binding
GABA receptor activity
protein heterodimerization activity
G protein-coupled neurotransmitter receptor activity involved in regulation of postsynaptic membrane potential
G protein-coupled neurotransmitter receptor activity involved in regulation of presynaptic membrane potential
extracellular matrix protein binding
signal transducer activity
transcription factor binding
G-protein coupled receptor
osteoblast differentiation
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
gamma-aminobutyric acid signaling pathway
negative regulation of cell proliferation
regulation of glutamate secretion
positive regulation of glutamate secretion
negative regulation of gamma-aminobutyric acid secretion
negative regulation of epinephrine secretion
negative regulation of dopamine secretion
response to nicotine
response to ethanol
negative regulation of synaptic transmission
synaptic transmission, GABAergic
regulation of postsynaptic membrane potential
positive regulation of growth hormone secretion
regulation of presynaptic membrane potential
neuron-glial cell signaling
negative regulation of adenylate cyclase activity
regulation of cAMP biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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