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Invitrogen
Versican Monoclonal Antibody (N351/23), APC
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FIGURE: 1 / 3
Versican Antibody (MA5-45623) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Versican monoclonal antibody (Product # MA5-45623) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue)... View More
Product Details
MA5-45623
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N351/23
Immunogen
Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45623 was sufficient for detection of Versican in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects >350kDa.
This antibody was formerly sold as clone S351-23.
Target Information
Versican is a member of the family of large aggregating proteoglycans (also including aggrecan, brevican, and neurocan). The protein shows wide tissue distribution which includes fibrous, articular, and elastic cartilages, as well as nervous, epidermal, arterial, and loose connective tissues. Versican V1 has been shown to enhance cell proliferation, and also induce cell transformation and protect cells from apoptosis. This was demonstrated through its ability to downregulate the expression of proapoptotic Bad. Versican's existence as a extracellular matrix protein in human airways has also lead to the discovery of an inflammatory role in asthmatics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chondroitin sulfate proteoglycan 2; chondroitin sulfate proteoglycan 2 (versican); chondroitin sulfate proteoglycan core protein; Chondroitin sulfate proteoglycan core protein 2; GHAP; Glial hyaluronate-binding protein; heart defect; hyaluronan-binding chondroitin sulfate proteoglycan; Large fibroblast proteoglycan; PG-M; PG-M core protein; PGM; unnamed protein product; V1 Neo; Versican core protein; Versican core protein precursor (Large fibroblast proteoglycan) (Chondroitin sulfate proteoglycan core protein 2) (PG-M); versican proteoglycan; Versican V0; WGN 1
Gene Aliases: 5430420N07Rik; 9430051N09; CSPG2; DPEAAE; ERVR; GHAP; hdf; NG2; PG-M; PG-M(V0); PG-M(V1); VCAN; WGN; WGN1
UniProt ID: (Human) P13611, (Rat) Q9ERB4
Entrez Gene ID: (Human) 1462, (Mouse) 13003, (Rat) 114122
calcium ion binding
protein binding
glycosaminoglycan binding
hyaluronic acid binding
protein tyrosine phosphatase activator activity
extracellular matrix structural constituent conferring compression resistance
carbohydrate binding
protein phosphatase binding
extracellular matrix structural constituent
low-density lipoprotein particle binding
extracellular matrix glycoprotein
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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