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Invitrogen

SHANK1/SHANK3 Monoclonal Antibody (N367/51), APC

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SHANK1/SHANK3 Monoclonal Antibody (N367/51), APC

Product Details

MA5-45673

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

N367/51

Immunogen

Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3.
3D Epitope / Immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932127

Product Specific Information

Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.

1 µg/mL of MA5-45673 was sufficient for detection of SHANK1/SHANK3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 190kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.

This antibody was formerly sold as clone S367-51.

Target Information

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation. {PubMed:21606927, PubMed:21795692, PubMed:23100419, PubMed:23897824, PubMed:24089484}.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; multidomain postsynaptic density protein; postsynaptic scaffold protein; proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; ProSAP2; SAPAP-interacting protein synamon; SH3 and multiple ankyrin repeat domains 1; SH3 and multiple ankyrin repeat domains protein 1; SH3 and multiple ankyrin repeat domains protein 3; SH3 and multiple ankyrin repeat domains protein 3 variant c-3; SH3 and multiple ankyrin repeat domains protein 3 variant c-4; SH3 and multiple ankyrin repeat domains-like protein 3 variant 1; SH3 and multiple ankyrin repeat domains-like protein 3 variant 2; SH3 and multiple ankyrin repeat domains-like protein 3 variant 3; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-3; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-2; SH3/ankyrin domain gene 1; SH3/ankyrin domain gene 3; shank postsynaptic density protein; Shank postsynaptic density protein 3a; Shank1; Shank3; shank3 postsynaptic density protein; shank3 splice variant II; shank3 splice variant III; shank3 splice variant IV; shank3e splice variant III; shank3e splice variant IV; Somatostatin receptor-interacting protein; SPANK-1; SPANK-2; SSTR-interacting protein; Synamon

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Gene Aliases: AI841104; DEL22q13.3; KIAA1650; PROSAP2; PSAP2; SCZD15; SHANK1; Shank1a; SHANK3; Shank3b; SHANK3c-3; SHANK3c-4; SPANK-1; SPANK-2; Spank1; SSTRIP; synamon

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UniProt ID: (Human) Q9Y566, (Human) Q9BYB0, (Rat) Q9WV48, (Mouse) D3YZU1, (Mouse) Q4ACU6, (Rat) Q9JLU4

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Entrez Gene ID: (Human) 50944, (Human) 85358, (Rat) 78957, (Mouse) 243961, (Mouse) 58234, (Rat) 59312

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Function(s)
G-protein coupled receptor binding protein binding SH3 domain binding receptor signaling complex scaffold activity GKAP/Homer scaffold activity somatostatin receptor binding ionotropic glutamate receptor binding identical protein binding macromolecular complex binding ankyrin repeat binding scaffold protein binding structural constituent of postsynaptic density actin binding zinc ion binding protein C-terminus binding protein complex binding protein self-association
Process(es)
MAPK cascade embryonic epithelial tube formation synapse assembly learning memory striatal medium spiny neuron differentiation adult behavior negative regulation of actin filament bundle assembly social behavior locomotory exploration behavior vocal learning negative regulation of cell volume positive regulation of long-term neuronal synaptic plasticity brain morphogenesis neuromuscular process controlling balance protein oligomerization positive regulation of synapse structural plasticity positive regulation of synaptic transmission, glutamatergic dendritic spine morphogenesis positive regulation of dendritic spine development regulation of dendritic spine morphogenesis vocalization behavior postsynaptic density assembly AMPA glutamate receptor clustering NMDA glutamate receptor clustering guanylate kinase-associated protein clustering regulation of long-term synaptic potentiation positive regulation of long-term synaptic potentiation positive regulation of glutamate receptor signaling pathway regulation of long term synaptic depression positive regulation of excitatory postsynaptic potential regulation of grooming behavior regulation of behavioral fear response positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity associative learning synapse organization cognition nervous system development long-term memory cell differentiation protein localization to synapse olfactory behavior habituation modulation of synaptic transmission determination of affect righting reflex synapse maturation protein-containing complex assembly AMPA selective glutamate receptor signaling pathway maintenance of postsynaptic density structure protein complex assembly cytoskeletal anchoring at plasma membrane regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
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