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Invitrogen
SHANK1/SHANK3 Monoclonal Antibody (N367/51), APC
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FIGURE: 1 / 3
SHANK1/SHANK3 Antibody (MA5-45673) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with SHANK1/SHANK3 monoclonal antibody (Product # MA5-45673) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,00... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45673
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N367/51
Immunogen
Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3.
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.
1 µg/mL of MA5-45673 was sufficient for detection of SHANK1/SHANK3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 190kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.
This antibody was formerly sold as clone S367-51.
Target Information
Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation. {PubMed:21606927, PubMed:21795692, PubMed:23100419, PubMed:23897824, PubMed:24089484}.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain specific protein; brain-specific protein; GKAP/SAPAP-interacting protein; multidomain postsynaptic density protein; postsynaptic density protein; postsynaptic scaffold protein; proline rich synapse associated protein 2; proline-rich synapse-associated protein 2; SAPAP-interacting protein synamon; SH3/ankyrin domain gene 1; SH3/ankyrin domain gene 3; shank postsynaptic density protein; Shank postsynaptic density protein 3a; shank3 postsynaptic density protein; somatostatin receptor-interacting protein; SPANK-1; SPANK-2; proSAP2; proline-rich synapse-associated protein 2; SSTR-interacting protein; synamon
Gene Aliases: DEL22q13.3; PROSAP2; PSAP2; SCZD15; Shank1a; SPANK-1; SPANK-2; Spank1; SSTRIP; synamon
UniProt ID: (Human) Q9Y566, (Human) Q9BYB0, (Rat) Q9WV48, (Mouse) D3YZU1, (Rat) Q9JLU4, (Mouse) Q4ACU6
Entrez Gene ID: (Human) 50944, (Human) 85358, (Rat) 78957, (Mouse) 243961, (Rat) 59312, (Mouse) 58234
G-protein coupled receptor binding
protein binding
SH3 domain binding
receptor signaling complex scaffold activity
GKAP/Homer scaffold activity
somatostatin receptor binding
ionotropic glutamate receptor binding
identical protein binding
macromolecular complex binding
ankyrin repeat binding
scaffold protein binding
structural constituent of postsynaptic density
zinc ion binding
receptor binding
long-term memory
associative learning
adult behavior
negative regulation of actin filament bundle assembly
social behavior
protein localization to synapse
olfactory behavior
habituation
modulation of synaptic transmission
synapse organization
neuromuscular process controlling balance
determination of affect
righting reflex
synapse maturation
dendritic spine morphogenesis
positive regulation of dendritic spine development
protein-containing complex assembly
vocalization behavior
AMPA selective glutamate receptor signaling pathway
maintenance of postsynaptic density structure
positive regulation of excitatory postsynaptic potential
MAPK cascade
embryonic epithelial tube formation
synapse assembly
learning or memory
learning
memory
locomotory behavior
gene expression
glial cell proliferation
striatal medium spiny neuron differentiation
exploration behavior
locomotory exploration behavior
locomotion
vocal learning
negative regulation of cell volume
regulation of synaptic plasticity
positive regulation of long-term neuronal synaptic plasticity
brain morphogenesis
sensory perception of touch
positive regulation of synapse structural plasticity
positive regulation of synaptic transmission, glutamatergic
long-term synaptic potentiation
long term synaptic depression
regulation of dendritic spine morphogenesis
neural precursor cell proliferation
postsynaptic density assembly
AMPA glutamate receptor clustering
NMDA glutamate receptor clustering
guanylate kinase-associated protein clustering
response to interleukin-17
regulation of postsynapse organization
regulation of long-term synaptic potentiation
positive regulation of long-term synaptic potentiation
positive regulation of glutamate receptor signaling pathway
regulation of long term synaptic depression
regulation of AMPA glutamate receptor clustering
regulation of grooming behavior
regulation of behavioral fear response
cognition
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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