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Invitrogen
Acrolein Monoclonal Antibody (2H2), PerCP
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FIGURE: 1 / 2
Acrolein Antibody (MA5-45763) in Flow
Flow Cytometry analysis using human neuroblastoma cells. Fixation involved 90% Methanol. Samples were incubated with Acrolein monoclonal antibody (Product # MA5-45763) at 1:50 for 30 min on ice, followed by Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
Product Details
MA5-45763
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2H2
Immunogen
Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:1,000 dilution of MA5-45763 was sufficient for detection of Acrolein in 2 µg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Acrolein is primarily used as an intermediate in the synthesis of acrylic acid and as a biocide. Acrolein may be formed from the breakdown of certain pollutants in outdoor air or from the burning of organic matter including tobacco, or fuels such as gasoline or oil. Acrolein is toxic to humans following inhalation, oral or dermal exposures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: 2-propenal; Acraldehyde; Acrolein; Acrylic aldehyde
Disclaimer
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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