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Bethyl Laboratories
MADD Polyclonal Antibody
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FIGURE: 1 / 2
MADD Antibody (A302-144A) in IHC (P)
Detection of human MADD by immunohistochemistry. Sample: FFPE sections of human breast carcinoma and colon carcinoma. Antibody: Affinity purified rabbit anti-MADD (Product # A302-144A) (Lot 1) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB. and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.
Product Details
A302-144A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1597 and 1647 of human MAP-kinase activating death domain.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
Tumor necrosis factor receptor 1 (TNFR1) contains a death receptor domain that mediates interactions involved in downstream signaling of TNF. A novel protein, MADD (mitogen activated protein (MAP)-kinase activating death domain protein) has recently been shown to associate with the death domain of TNFR1 through its own C-terminal death domain. This interaction is critical for TNFR1 signal generation. Overexpression of MADD activates the MAP (Mitogen Activated Protein) kinases extracellular signal-related kinase (ERK) and c-Jun N-terminal kinase (JNK) and induces the phosphorylation of cytosolic phospholipase A2. This discovery suggests that MADD links tumor necrosis factor TNF and MAP kinase activation and arachidonic acid release. MADD has a 98% homology with another protein, differentially expressed in neoplasmic vs. normal cells (DENN). DENN is a substrate for JNK3, and is important in the hypoxia and stress induced intracellular signalling pathways. MADD also has a 93% sequence conservation with a GDP/GTP exchange protein, Rab3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Differentially expressed in normal and neoplastic cells; IG20-SV4; signaling protein; Insulinoma glucagonoma clone 20; Insuloma-Glucagonoma protein 20; KIAA0358; MAP kinase-activating death domain protein; Rab3 GDP/GTP exchange factor; Rab3 GDP/GTP exchange protein; Rab3GEP; RabGEF; signaling protein; unnamed protein product
Gene Aliases: DEEAH; DENN; IG20; KIAA0358; MADD; NEDDISH; RAB3GEP; RabGEF
UniProt ID: (Human) Q8WXG6
Entrez Gene ID: (Human) 8567
apoptotic process
cell surface receptor signaling pathway
regulation of Rab protein signal transduction
regulation of apoptotic process
positive regulation of MAPK cascade
regulation of cell cycle
execution phase of apoptosis
regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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