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MBD1 Polyclonal Antibody
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FIGURE: 1 / 2
MBD1 Antibody (600-401-X42) in WB
Western Blot results of Rabbit anti-MBD1 antibody. Lane 1: Nuclear extracts of HeLa cells. Load: 40 µg per lane. Primary Antibody: anti-MBD1 antibody at 1:500 overnight at 4°C. Block: TBS-Tween/5% BLOTTO. Secondary Antibody: anti-rabbit HRP at 1:10,000 for 1hr at RT.
Product Details
600-401-X42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-MBD1 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminus of human MBD1.
Conjugate
Form
Liquid
Concentration
0.7 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.05% sodium azide, 0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA. It is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). MBD1 acts as a transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CXXC-type zinc finger protein 3; Methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; PCM1; protein containing MBD 1; Protein containing methyl-CpG-binding domain 1; the regulator of fibroblast growth factor 2 (FGF-2) transcription; unnamed protein product
Gene Aliases: CXXC3; MBD1; PCM1; RFT
UniProt ID: (Human) Q9UIS9, (Mouse) Q9Z2E2
Entrez Gene ID: (Human) 4152, (Mouse) 17190
DNA methylation
transcription, DNA-templated
regulation of transcription, DNA-templated
negative regulation of gene expression
negative regulation of astrocyte differentiation
negative regulation of transcription from RNA polymerase II promoter
methylation-dependent chromatin silencing
transcription from RNA polymerase II promoter
negative regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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