There is one major form (long chain) and two minor forms (short chain and medium chain) of MCP-4 produced by differential signal peptide cleavage. Thus, the resulting MW of secreted MCP-4 ranges between ~8-9kD. Western blot analysis of recombinant MCP-4/CCL13 protein detects an ~17kD band, likely corresponding to a dimer of MCP-4.
The M809 MCP-4 antibody (clone 3G4) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M810 (biotinylated conjugate of clone 8C12). The M809 MCP-4 antibody (biotinylated conjugate of clone 3G4) has also been used successfully in a sandwich ELISA as a detection antibody when paired with M810 (clone 8C12) as the coating antibody.
Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The gene encoding MCP-4/CCL13 is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. MCP-4/CCL13 signals through CCR2B and CCR3 receptors, and plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. It be also involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis, and may also play a role in monocyte attraction in tissues chronically exposed to exogenous pathogens.
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Protein Aliases: C-C Motif Chemokine 13; C-C motif chemokine 13, long chain; C-C motif chemokine 13, medium chain; C-C motif chemokine 13, short chain; Chemokine (C-C Motif) Ligand 13; CK-Beta-10; CKb10; MCP-4; MCP4; Monocyte Chemoattractant Protein 4; Monocyte chemotactic protein 4; Monocyte chemotatic protein 4; NCC-1; NCC1; New CC Chemokine 1; SCYA13; SCYL1; Small inducible cytokine subfamily A (Cys-Cys) member 13; small inducible cytokine subfamily A (Cys-Cys), member 13; Small-inducible cytokine A13
Gene Aliases: CCL13; CKb10; MCP-4; MCP4; NCC-1; NCC1; SCYA13; SCYL1
UniProt ID: (Human) Q99616
Entrez Gene ID: (Human) 6357