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Invitrogen
MCU Polyclonal Antibody
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FIGURE: 1 / 2
MCU Antibody (PA5-103754) in IHC (P)
Immunohistochemistry analysis of MCU in human ovarian cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MCU polyclonal antibody (Product # PA5-103754) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-103754
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MCU(Accession Q8NE86), corresponding to amino acid residues S92-D142.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CCDC109A.
Target Information
Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex). Activity is regulated by MICU1 and MICU2. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. participates in mitochondrial permeability transition during ischemia-reperfusion injury. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Involved in antigen processing and presentation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium uniporter protein, mitochondrial; coiled-coil domain containing 109A; Coiled-coil domain-containing protein 109A; HsMCU
Gene Aliases: 2010012O16Rik; AV064928; C10orf42; CCDC109A; D130073L02Rik; Gm64; MCU
UniProt ID: (Human) Q8NE86, (Mouse) Q3UMR5
Entrez Gene ID: (Human) 90550, (Mouse) 215999
mitochondrial calcium ion transport
calcium-mediated signaling
positive regulation of insulin secretion
protein complex oligomerization
calcium ion transmembrane import into mitochondrion
glucose homeostasis
positive regulation of mitochondrial calcium ion concentration
transport
ion transport
calcium ion transport
mitochondrial calcium ion homeostasis
calcium ion transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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