Search
Rockland
MDA5 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 3
MDA5 Antibody (600-401-CS7) in ICC/IF
Immunofluorescence Microscopy of MDA5 antibody. Tissue: Human lymph node cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: MDA5 antibody at 20 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: MDA5 as red fluorescent signal.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
600-401-CS7
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-MDA5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the internal region of human MDA5.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CADM-140 autoantigen; clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; helicard; helicase; helicase with 2 CARD domains; interferon induced with helicase C domain protein 1; melanoma differentiation-associated gene 5; melanoma differentiation-associated protein 5; MGC133047; murabutide down-regulated protein; RIG-I-like receptor 2; RNA helicase; RNA helicase-DEAD box protein 116; RNA helicase; MDA-5; RNA-dependent ATPase; unnamed protein product
Gene Aliases: 9130009C22Rik; AGS7; Helicard; Hlcd; IDDM19; IMD95; MDA-5; MDA5; RLR-2; SGMRT1
UniProt ID: (Human) Q9BYX4, (Mouse) Q8R5F7
Entrez Gene ID: (Human) 64135, (Mouse) 71586
DNA binding
RNA binding
RNA helicase activity
double-stranded RNA binding
single-stranded RNA binding
protein binding
ATP binding
zinc ion binding
hydrolase activity
ATPase activity
protein domain specific binding
pattern recognition receptor activity
identical protein binding
ribonucleoprotein complex binding
cytoplasmic pattern recognition receptor signaling pathway
signal transduction
detection of virus
response to virus
protein sumoylation
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
regulation of type III interferon production
MDA-5 signaling pathway
negative regulation of viral genome replication
innate immune response
protein oligomerization
defense response to virus
positive regulation of response to cytokine stimulus
cellular response to exogenous dsRNA
cellular response to virus
antiviral innate immune response
mRNA transcription
type I interferon signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support