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Invitrogen
MDFI Polyclonal Antibody
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Product Details
PA5-89376
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MDFI (NP_0055771)
Conjugate
Form
Liquid
Concentration
0.06 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MYQVSGQRPS GCDAPYGAPS AAPGPAQTLS LLPGLEVVTG STHPAEAAPE EGSLEEAATP MPQGNGPGIP QGLDSTDLDV PTEAVTCQPQ GNPLGCTPLL PNDSGHPSEL GGTRRAGNGA LGGPKAHRKL QTHPSLASQG SKKSKSSSKS TTSQIPLQAQ; Positive Samples: Mouse heart, Rat heart; Cellular Location: Cytoplasm, Nucleus
Target Information
MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. MDFI an also interfere with the DNA-binding activity of Myod family members and plays an important role in trophoblast and chondrogenic differentiation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: inhibitor of MyoD family a; MyoD family inhibitor; Myogenic repressor I-mf
Gene Aliases: I-MF; I-mfa; MDFI; RGD1560271
UniProt ID: (Human) Q99750, (Mouse) P70331
Entrez Gene ID: (Human) 4188, (Mouse) 17240, (Rat) 501097
negative regulation of transcription from RNA polymerase II promoter
activation of JUN kinase activity
multicellular organism development
dorsal/ventral axis specification
regulation of Wnt signaling pathway
cell differentiation
negative regulation of Wnt signaling pathway
negative regulation of DNA binding
embryonic skeletal system morphogenesis
trophoblast giant cell differentiation
embryo development
cytoplasmic sequestering of transcription factor
negative regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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