Proteintech

MDH2 Polyclonal Antibody

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MDH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 15462-1-AP) (MDH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MDH2 Antibody in Western Blot (WB) — Mouse lung tissue were subjected to SDS PAGE followed by western blot with 15462-1-AP ( MDH2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

MDH2 Antibody in Immunoprecipitation (IP) — IP result of anti-MDH2 (IP:15462-1-AP, 4ug; Detection:15462-1-AP 1:300) with mouse lung tissue lysate 3600ug.

Product Details

15462-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Plant

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MDH2 Fusion Protein Ag7750 (1-338 aa encoded by BC001917)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MLSALARPV SAALRRSFST SAQNNAKVAV LGASGGIGQP LSLLLKNSPL VSRLTLYDIA HTPGVAADLS HIETKAAVKG YLGPEQLPDC LKGCDVVVIP AGVPRKPGMT RDDLFNTNAT IVATLTAACA QHCPEAMICV IANPVNSTIP ITAEVFKKHG VYNPNKIFGV TTLDIVRANT FVAELKGLDP ARVNVPVIGG HAGKTIIPLI SQCTPKVDFP QDQLTALTGR IQEAGTEVVK AKAGAGSATL SMAYAGARFV FSLVDAMNGK EGVVECSFVK SQETECTYFS TPLLLGKKGI EKNLGIGKVS SFEEKMISDA IPELKASIKK GEDFVKTLK (1-338 aa encoded by BC001917 )

Target Information

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of asparagine biosynthesis pathway in Siniperca chuatsi rhabdovirus proliferation.

Frontiers in microbiology

Li F,Fu X,Luo X,Lin Q,Liang H,Niu Y,Liu L,Li N

Tue Apr 18 00:00:00 EDT 2023

AMPK-dependent phosphorylation of MTFR1L regulates mitochondrial morphology.

Science advances

Tilokani L,Russell FM,Hamilton S,Virga DM,Segawa M,Paupe V,Gruszczyk AV,Protasoni M,Tabara LC,Johnson M,Anand H,Murphy MP,Hardie DG,Polleux F,Prudent J

Fri Nov 11 00:00:00 EST 2022

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging.

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Yuan M,Wang Y,Wen J,Jing F,Zou Q,Pu Y,Pan T,Cai Z

Sat Oct 01 00:00:00 EDT 2022

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Nature communications

Wang XH,Xu S,Zhou XY,Zhao R,Lin Y,Cao J,Zang WD,Tao H,Xu W,Li MQ,Zhao SM,Jin LP,Zhao JY

Tue Jun 08 00:00:00 EDT 2021

The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.

iScience

Sinha T,Naash MI,Al-Ubaidi MR

Fri Apr 24 00:00:00 EDT 2020

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

The Journal of experimental medicine

Liu M,Wang Y,Yang C,Ruan Y,Bai C,Chu Q,Cui Y,Chen C,Ying G,Li B

Mon Mar 02 00:00:00 EST 2020

Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure.

Biochimica et biophysica acta. Molecular basis of disease

Guo Q,Zhang Y,Zhang S,Jin J,Pang S,Wu X,Zhang W,Bi X,Zhang Y,Zhang Q,Jiang F

Sun Mar 01 00:00:00 EST 2020

Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E₂ alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.

International journal of radiation biology

Sun Y,Shi Z,Wang Y,Tang C,Liao Y,Yang C,Cai P

Sat Dec 01 00:00:00 EST 2018

Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E₂ alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.

International journal of radiation biology

Sun Y,Shi Z,Wang Y,Tang C,Liao Y,Yang C,Cai P

Sat Dec 01 00:00:00 EST 2018

LONP1 Is Required for Maturation of a Subset of Mitochondrial Proteins, and Its Loss Elicits an Integrated Stress Response.

Molecular and cellular biology

Zurita Rendón O,Shoubridge EA

Mon Oct 15 00:00:00 EDT 2018

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Journal of ethnopharmacology

Wu L,Cao KX,Ni ZH,Li WD,Chen ZP,Cheng HB,Liu X

Fri Aug 10 00:00:00 EDT 2018

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Journal of ethnopharmacology

Wu L,Cao KX,Ni ZH,Li WD,Chen ZP,Cheng HB,Liu X

Fri Aug 10 00:00:00 EDT 2018

Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase.

FEBS letters

Rhein VF,Carroll J,Ding S,Fearnley IM,Walker JE

Thu Jun 01 00:00:00 EDT 2017

Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase.

FEBS letters

Rhein VF,Carroll J,Ding S,Fearnley IM,Walker JE

Thu Jun 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: malate dehydrogenase 2, NAD (mitochondrial); Malate dehydrogenase, mitochondrial; mitochondrial malate dehydrogenase 2, NAD; testicular tissue protein Li 120

Gene Aliases: M-MDH; MDH; Mdh-2; MDH2; MGC:3559; Mor-1; MOR1

UniProt ID: (Human) P40926, (Rat) P04636, (Mouse) P08249

Entrez Gene ID: (Human) 4191, (Rat) 81829, (Mouse) 17448

Molecular Function: dehydrogenase