|Immunoprecipitation (IP)||1-5 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
|Western Blot (WB)||See 4 publications below|
|Miscellaneous PubMed (MISC)||See 5 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 6 publications below|
|Immunohistochemistry (IHC)||See 8 publications below|
|Published species||Bacteria, Guinea pig, Human, Rat|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Synthetic peptide derived from the n-terminal region of human MDM2|
|Storage buffer||PBS, pH 7.4, with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody recognizes the ~90 kDa (apparent MW) MDM2 protein. Also recognizes isoforms at ~57 and ~74/76 kDa. The epitope recognized by this antibody is located within amino acids 26-169 of the human protein. Positive controls: OsA-CL, MCF-7, HeLa cell lysates. HOS cells can be used for negative control. Staining of formalin fixed, paraffin embedded tissue requires heat induced epitope retrieval pretreatment.
The mdm2 (murine double minute 2) proto-oncogene was originally identified as an amplified gene in a mouse tumor cell line. Subsequently, overexpression of MDM2 was shown to produce tumors in athymic mice. In a separate set of studies, the 90 kDa MDM2 was found to bind and inactivate the transcriptional activity of the p53 protein. p53 was also identified as the major target for MDM2 during embryonic development by virtue of the fact that the lethal effects produced by knocking out mdm2 could be reversed by the simultaneous deletion of p53. Interestingly, the mdm2 gene itself is a transcriptional target for p53, and induction of p53 transcriptional activity results in increased MDM2 mRNA and protein levels. Therefore, it appears that a MDM2«p53 feedback loop serves to keep the growth suppressive functions of p53 in check during the normal cell cycle. In addition, recent studies have implicated MDM2 in regulating cell proliferation via p53-independent pathways. This is based on evidence that MDM2 can interact with Rb, E2F-1 and DP1.
Because MDM2 can bind to and inactivate the transcriptional activity of p53, overexpression of MDM2 protein has been detected in a variety of human tumors, and appears to result from gene amplification, increased transcript level, and enhanced translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2 proto-oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2
Gene Aliases: ACTFS; hdm2; HDMX; MDM2
UniProt ID: (Human) Q00987
Entrez Gene ID: (Human) 4193
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