A suggested positive control is human testis tissue lysate.
PA5-20571 can be used with blocking peptide PEP-0691.
The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for activation of polymerase II transcribed genes. MED4 appears to be a core Mediator subunit and is found in nearly all Mediator preparations (Sato et al., 2004).
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Protein Aliases: Activator-recruited cofactor 36 kDa component; ARC36; DRIP36; Mediator complex subunit 4; Mediator of RNA polymerase II transcription subunit 4; mediator of RNA polymerase II transcription, subunit 4 homolog; mediator, 34-kD subunit, homolog; p36 TRAP/SMCC/PC2 subunit; RP11-90M2.2, ARC36, DRIP36, HSPC126, TRAP36, VDRIP; TRAP/SMCC/PC2 subunit p36; TRAP/SMCC/PC2 subunit p36 subunit; vitamin D receptor interacting protein; vitamin D receptor-interacting protein, 36-kD; Vitamin D3 receptor-interacting protein complex 36 kDa component
Gene Aliases: 2410046H15Rik; ARC36; DRIP36; HSPC126; MED4; TRAP36; Vdirp; VDRIP