|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (coupled with KLH) derived from aminoacid sequence 33-53 of human MAPK Kinase 1. The immunogen sequence is highly conserved within species (human, mouse, rat, hamster, bovine etc.).|
|Storage buffer||PBS with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MEK (MAPKK, kinase of mitogen-activated protein kinase) is the medium one of three components of MAP kinase cascade - an important signaling pathway that regulates cell growth and differentiation. Raf (MAPKKK) activates MEK 1 and 2 via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK (MAPK, mitogen-activated protein kinase). This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade. Phosphorylated ERK is capable of translocating to the nucleus, where it regulates gene expression by activating transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.