Invitrogen

METT10D Polyclonal Antibody

View all (18) METT10D antibodies

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METT10D Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of METT10D in C6 cells using a METT10D Polyclonal antibody (Product # PA5-120402). Blue: DAPI for nuclear staining.

METT10D Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of METT10D in C6 cells using a METT10D Polyclonal antibody (Product # PA5-120402). Blue: DAPI for nuclear staining.

METT10D Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of METT10D in C6 cells using a METT10D Polyclonal antibody (Product # PA5-120402). Blue: DAPI for nuclear staining.

METT10D Antibody in Western Blot (WB) — Western Blot analysis of METT10D in various cell lines using a METT10D Polyclonal antibody (Product # PA5-120402) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 3s.

METT10D Antibody in Western Blot (WB) — Western blot analysis of METT10D in LNCaP cells using a METT10D Polyclonal antibody (Product # PA5-120402) at a dilution of 1:1000.

Product Details

PA5-120402

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500 - 1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50 - 1:200	-
Immunoprecipitation (IP)	1:50 - 1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human METTL16 (NP_076991.3). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	3.18 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.01% thimerosal
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2913974

Product Specific Information

Positive test controls include: HeLa, A-549, NIH/3T3, C6, LNCaP, Rat testis.

Target Information

METT10D is a probable methyltransferase.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: methyltransferase 10 domain containing; Methyltransferase 10 domain-containing protein; Methyltransferase-like protein 16; N6-adenosine-methyltransferase METTL16; putative methyltransferase METT10D; RNA N6-adenosine-methyltransferase METTL16; U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase

Gene Aliases: 2610100D03Rik; 2810013M15Rik; A830095F14Rik; AI846133; METT10D; METTL16

UniProt ID: (Human) Q86W50, (Mouse) Q9CQG2

Entrez Gene ID: (Human) 79066, (Mouse) 67493

Molecular Function: methyltransferase

rRNA base methylation biological_process methylation

Process(es)

poly(A) RNA binding 23S rRNA (adenine(1618)-N(6))-methyltransferase activity rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity