Proteintech

METTL1 Polyclonal Antibody

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METTL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 14994-1-AP (METTL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

METTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas using 14994-1-AP (METTL1 antibody) at dilution of 1:100 (under 40x lens).

METTL1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

METTL1 Antibody in Immunoprecipitation (IP) — IP result of anti-METTL1 (IP:14994-1-AP, 4ug; Detection:14994-1-AP 1:300) with A2780 cells lysate 3600 ug.

Product Details

14994-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	METTL1 Fusion Protein Ag6980 (1-276 aa encoded by BC000550) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAAETRNVA GAEAPPPQKR YYRQRAHSNP MADHTLRYPV KPEEMDWSEL YPEFFAPLTQ NQSHDDPKDK KEKRAQAQVE FADIGCGYGG LLVELSPLFP DTLILGLEIR VKVSDYVQDR IRALRAAPAG GFQNIACLRS NAMKHLPNFF YKGQLTKMFF LFPDPHFKRT KHKWRIISPT LLAEYAYVLR VGGLVYTITD VLELHDWMCT HFEEHPLFER VPLEDLSEDP VVGHLGTSTE EGKKVLRNGG KNFPAIFRRI QDPVLQAVTS QTSLPGH (1-276 aa encoded by BC000550)

Target Information

This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mettl1/Wdr4-Mediated m⁷G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.

Molecular cell

Lin S,Liu Q,Lelyveld VS,Choe J,Szostak JW,Gregory RI

Thu Jul 19 00:00:00 EDT 2018

tRNA modifying enzymes, NSUN2 and METTL1, determine sensitivity to 5-fluorouracil in HeLa cells.

PLoS genetics

Okamoto M,Fujiwara M,Hori M,Okada K,Yazama F,Konishi H,Xiao Y,Qi G,Shimamoto F,Ota T,Temme A,Tatsuka M

Mon Sep 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: D1075-like gene product; methyltransferase like 1; methyltransferase-like 1; Methyltransferase-like protein 1; METTL1; miRNA (guanine-N(7)-)-methyltransferase; mRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine(46)-N(7))-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase

Gene Aliases: 2810012D02Rik; C12orf1; METTL1; TRM8; TRMT8; YDL201w

UniProt ID: (Human) Q9UBP6, (Mouse) Q9Z120

Entrez Gene ID: (Human) 4234, (Rat) 679091, (Mouse) 17299

Molecular Function: RNA processing factor