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METTL1 Polyclonal Antibody

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Antibody Testing Data (11)

METTL1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

METTL1 Antibody (14994-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 14994-1-AP (METTL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

METTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

METTL1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14994-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

METTL1 Fusion Protein Ag6980 (1-276 aa encoded by BC000550)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAAETRNVA GAEAPPPQKR YYRQRAHSNP MADHTLRYPV KPEEMDWSEL YPEFFAPLTQ NQSHDDPKDK KEKRAQAQVE FADIGCGYGG LLVELSPLFP DTLILGLEIR VKVSDYVQDR IRALRAAPAG GFQNIACLRS NAMKHLPNFF YKGQLTKMFF LFPDPHFKRT KHKWRIISPT LLAEYAYVLR VGGLVYTITD VLELHDWMCT HFEEHPLFER VPLEDLSEDP VVGHLGTSTE EGKKVLRNGG KNFPAIFRRI QDPVLQAVTS QTSLPGH (1-276 aa encoded by BC000550)

Target Information

This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

METTL1/WDR4-mediated tRNA m⁷G modification and mRNA translation control promote oncogenesis and doxorubicin resistance.

Oncogene

Wang Z,Yu P,Zou Y,Ma J,Han H,Wei W,Yang C,Zheng S,Guo S,Wang J,Liu L,Lin S

Thu Jun 01 00:00:00 EDT 2023

Structural basis of regulated m⁷G tRNA modification by METTL1-WDR4.

Nature

Li J,Wang L,Hahn Q,Nowak RP,Viennet T,Orellana EA,Roy Burman SS,Yue H,Hunkeler M,Fontana P,Wu H,Arthanari H,Fischer ES,Gregory RI

Sun Jan 01 00:00:00 EST 2023

A Comprehensive Analysis of METTL1 to Immunity and Stemness in Pan-Cancer.

Frontiers in immunology

Gao Z,Xu J,Zhang Z,Fan Y,Xue H,Guo X,Deng L,Wang S,Zhao R,Zhang P,Li G

Tue Apr 19 00:00:00 EDT 2022

N⁷-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma tumorigenesis via the RPTOR/ULK1/autophagy axis.

Nature communications

Han H,Yang C,Ma J,Zhang S,Zheng S,Ling R,Sun K,Guo S,Huang B,Liang Y,Wang L,Chen S,Wang Z,Wei W,Huang Y,Peng H,Jiang YZ,Choe J,Lin S

Fri Mar 18 00:00:00 EDT 2022

Aberrant translation regulated by METTL1/WDR4-mediated tRNA N7-methylguanosine modification drives head and neck squamous cell carcinoma progression.

Cancer communications (London, England)

Chen J,Li K,Chen J,Wang X,Ling R,Cheng M,Chen Z,Chen F,He Q,Li S,Zhang C,Jiang Y,Chen Q,Wang A,Chen D

Tue Mar 01 00:00:00 EST 2022

Aberrant translation regulated by METTL1/WDR4-mediated tRNA N7-methylguanosine modification drives head and neck squamous cell carcinoma progression.

Cancer communications (London, England)

Chen J,Li K,Chen J,Wang X,Ling R,Cheng M,Chen Z,Chen F,He Q,Li S,Zhang C,Jiang Y,Chen Q,Wang A,Chen D

Tue Mar 01 00:00:00 EST 2022

SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells.

BMC biology

Yang Y,Qiu R,Zhao S,Shen L,Tang B,Weng Q,Xu Z,Zheng L,Chen W,Shu G,Wang Y,Zhao Z,Chen M,Ji J

Tue Dec 27 00:00:00 EST 2022

METTL1 promotes neuroblastoma development through m⁷G tRNA modification and selective oncogenic gene translation.

Biomarker research

Huang Y,Ma J,Yang C,Wei P,Yang M,Han H,Chen HD,Yue T,Xiao S,Chen X,Li Z,Tang Y,Luo J,Lin S,Huang L

Wed Sep 07 00:00:00 EDT 2022

METTL1 promotes neuroblastoma development through m⁷G tRNA modification and selective oncogenic gene translation.

Biomarker research

Huang Y,Ma J,Yang C,Wei P,Yang M,Han H,Chen HD,Yue T,Xiao S,Chen X,Li Z,Tang Y,Luo J,Lin S,Huang L

Wed Sep 07 00:00:00 EDT 2022

METTL1 promotes neuroblastoma development through m⁷G tRNA modification and selective oncogenic gene translation.

Biomarker research

Huang Y,Ma J,Yang C,Wei P,Yang M,Han H,Chen HD,Yue T,Xiao S,Chen X,Li Z,Tang Y,Luo J,Lin S,Huang L

Wed Sep 07 00:00:00 EDT 2022

METTL1 promotes neuroblastoma development through m⁷G tRNA modification and selective oncogenic gene translation.

Biomarker research

Huang Y,Ma J,Yang C,Wei P,Yang M,Han H,Chen HD,Yue T,Xiao S,Chen X,Li Z,Tang Y,Luo J,Lin S,Huang L

Wed Sep 07 00:00:00 EDT 2022

METTL1 drives tumor progression of bladder cancer via degrading ATF3 mRNA in an m⁷G-modified miR-760-dependent manner.

Cell death discovery

Xie H,Wang M,Yu H,Wang H,Ding L,Wang R,Luo W,Lu Z,Zheng Q,Ren L,Zhou Z,Su W,Xia L,Li G

Thu Nov 17 00:00:00 EST 2022

METTL1 drives tumor progression of bladder cancer via degrading ATF3 mRNA in an m⁷G-modified miR-760-dependent manner.

Cell death discovery

Xie H,Wang M,Yu H,Wang H,Ding L,Wang R,Luo W,Lu Z,Zheng Q,Ren L,Zhou Z,Su W,Xia L,Li G

Thu Nov 17 00:00:00 EST 2022

METTL1-mediated m⁷G modification of Arg-TCT tRNA drives oncogenic transformation.

Molecular cell

Orellana EA,Liu Q,Yankova E,Pirouz M,De Braekeleer E,Zhang W,Lim J,Aspris D,Sendinc E,Garyfallos DA,Gu M,Ali R,Gutierrez A,Mikutis S,Bernardes GJL,Fischer ES,Bradley A,Vassiliou GS,Slack FJ,Tzelepis K,Gregory RI

Thu Aug 19 00:00:00 EDT 2021

N⁷-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.

Molecular cell

Dai Z,Liu H,Liao J,Huang C,Ren X,Zhu W,Zhu S,Peng B,Li S,Lai J,Liang L,Xu L,Peng S,Lin S,Kuang M

Thu Aug 19 00:00:00 EDT 2021

N⁷-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.

Molecular cell

Dai Z,Liu H,Liao J,Huang C,Ren X,Zhu W,Zhu S,Peng B,Li S,Lai J,Liang L,Xu L,Peng S,Lin S,Kuang M

Thu Aug 19 00:00:00 EDT 2021

N⁷-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.

Molecular cell

Dai Z,Liu H,Liao J,Huang C,Ren X,Zhu W,Zhu S,Peng B,Li S,Lai J,Liang L,Xu L,Peng S,Lin S,Kuang M

Thu Aug 19 00:00:00 EDT 2021

N⁷-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.

Molecular cell

Dai Z,Liu H,Liao J,Huang C,Ren X,Zhu W,Zhu S,Peng B,Li S,Lai J,Liang L,Xu L,Peng S,Lin S,Kuang M

Thu Aug 19 00:00:00 EDT 2021

Methyltransferase-like 1 regulates lung adenocarcinoma A549 cell proliferation and autophagy via the AKT/mTORC1 signaling pathway.

Oncology letters

Wang C,Wang W,Han X,Du L,Li A,Huang G

Thu Apr 01 00:00:00 EDT 2021

Prognostic role of METTL1 in glioma.

Cancer cell international

Li L,Yang Y,Wang Z,Xu C,Huang J,Li G

Sat Nov 27 00:00:00 EST 2021

Prognostic role of METTL1 in glioma.

Cancer cell international

Li L,Yang Y,Wang Z,Xu C,Huang J,Li G

Sat Nov 27 00:00:00 EST 2021

METTL1/WDR4-mediated m⁷G tRNA modifications and m⁷G codon usage promote mRNA translation and lung cancer progression.

Molecular therapy : the journal of the American Society of Gene Therapy

Ma J,Han H,Huang Y,Yang C,Zheng S,Cai T,Bi J,Huang X,Liu R,Huang L,Luo Y,Li W,Lin S

Wed Dec 01 00:00:00 EST 2021

Dynamic methylome of internal mRNA N⁷-methylguanosine and its regulatory role in translation.

Cell research

Malbec L,Zhang T,Chen YS,Zhang Y,Sun BF,Shi BY,Zhao YL,Yang Y,Yang YG

Fri Nov 01 00:00:00 EDT 2019

Mettl1/Wdr4-Mediated m⁷G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.

Molecular cell

Lin S,Liu Q,Lelyveld VS,Choe J,Szostak JW,Gregory RI

Thu Jul 19 00:00:00 EDT 2018

Mettl1/Wdr4-Mediated m⁷G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.

Molecular cell

Lin S,Liu Q,Lelyveld VS,Choe J,Szostak JW,Gregory RI

Thu Jul 19 00:00:00 EDT 2018

tRNA modifying enzymes, NSUN2 and METTL1, determine sensitivity to 5-fluorouracil in HeLa cells.

PLoS genetics

Okamoto M,Fujiwara M,Hori M,Okada K,Yazama F,Konishi H,Xiao Y,Qi G,Shimamoto F,Ota T,Temme A,Tatsuka M

Mon Sep 01 00:00:00 EDT 2014

tRNA modifying enzymes, NSUN2 and METTL1, determine sensitivity to 5-fluorouracil in HeLa cells.

PLoS genetics

Okamoto M,Fujiwara M,Hori M,Okada K,Yazama F,Konishi H,Xiao Y,Qi G,Shimamoto F,Ota T,Temme A,Tatsuka M

Mon Sep 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: D1075-like gene product; methyltransferase like 1; methyltransferase-like 1; Methyltransferase-like protein 1; METTL1; miRNA (guanine-N(7)-)-methyltransferase; mRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine(46)-N(7))-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase

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Gene Aliases: 2810012D02Rik; C12orf1; METTL1; TRM8; TRMT8; YDL201w

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UniProt ID: (Human) Q9UBP6, (Mouse) Q9Z120

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Entrez Gene ID: (Human) 4234, (Rat) 679091, (Mouse) 17299

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Function(s)

tRNA binding protein binding tRNA (guanine-N7-)-methyltransferase activity RNA binding methyltransferase activity transferase activity RNA processing factor

Process(es)

tRNA modification tRNA methylation RNA (guanine-N7)-methylation tRNA processing methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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METTL1 Polyclonal Antibody

METTL1 Antibody (14994-1-AP) in ICC/IF

Product Details

14994-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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