Invitrogen

METTL3 Polyclonal Antibody

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Datasheet

Protocols

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METTL3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of METTL3 in U-2 OS cells using a METTL3 Polyclonal antibody (Product # PA5-121190). Blue: DAPI for nuclear staining.

METTL3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of METTL3 in human esophageal cancer using a Polyclonal antibody (Product # PA5-121190) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..

METTL3 Antibody in Western Blot (WB) — Western Blot analysis of METTL3 in HT-1080 cells using a METTL3 Polyclonal antibody (Product # PA5-121190) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 1s.

Product Details

PA5-121190

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500 - 1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50 - 1:200	-
Immunoprecipitation (IP)	1:20 - 1:50	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.86 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.05% ProClin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2914762

Product Specific Information

Positive test controls include: 293T, HT-1080, NIH/3T3, Mouse testis, Mouse brain, Rat spleen. The target is usually found in the following locations: Nucleus speckle.

Target Information

METTL3 is the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; hMETTL3; m6a methyltransferase; methyltransferase-like 3; Methyltransferase-like protein 3; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; MT-A70; N6-adenosine-methyltransferase 70 kDa subunit; N6-adenosine-methyltransferase catalytic subunit; N6-adenosine-methyltransferase subunit METTL3

Gene Aliases: 2310024F18Rik; IME4; M6A; METTL3; MT-A70; MTA70; Spo8

UniProt ID: (Human) Q86U44, (Mouse) Q8C3P7

Entrez Gene ID: (Human) 56339, (Mouse) 56335, (Rat) 361035

Molecular Function: RNA metabolism protein RNA methyltransferase nucleic acid metabolism protein